IV型胶原酶在不同转移潜能的前列腺癌细胞系中的表达及活性分析

目的:探讨前列腺癌细胞转移潜能与IV型胶原酶基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)的关系。方法:应用免疫组织化学SABC法,研究IV型胶原酶在不同转移潜能的前列腺癌细胞株LNCaP、C4-2B中表达变化。同时采用明胶酶谱法对这2种前列腺癌细胞系分泌的IV型胶原酶活性进行分析。结果:①免疫组织化学染色显示,LNCaP、C4-2B细胞中均有MMP-2、MMP-9表达,主要表达于细胞质中,C4-2B细胞表达明显强于LNCaP(P<0.01)。②人前列腺癌细胞LNCaP的条件培养液中仅能检测到极低的MMP-2和MMP-9活性,酶谱条带的积分A值分别为0.89±0.02和0.86±0.01,主要以酶原形式存在,而C4-2B细胞则表现较强的MMP-2、MMP-9活性,积分A值分别为96.32±4.36和33.89±1.84,其分泌的MMP-2则以活性形式为主,其分泌量显著高于LNCaP细胞(P<0.01)。结论:前列腺癌细胞中IV型胶原酶的表达和分泌,有助于癌细胞的侵袭和转移;前列腺癌细胞的侵袭转移潜能与其产生IV型胶原酶的能力密切相关。

Type IV Collagenase Expression in Human Prostate Carcinoma Cell Lines with Different Metastasis Potentials

Objective: To investigate the correlation between type IV collagenase expression and metastatic potential of human prostate carcinoma cells.Methods: Human prostate carcinoma cell lines(LNCaP and C4-2B) with different metastatic potentials were selected.Using SABC immunocytochemical staining and zymographic analysis,we detected the difference of type IV collagenase expression and activity between the two cell lines.Results: The expression of type IV collagenase(MMP-2,MMP-9) could be detected in both LNCaP and C4-2B,mainly in cytoplasm,much higher in C4-2B than in LNCaP(P<(0.01)).The activities of type IV collagenase in the conditioned medium of prostatic carcinoma cell LNCaP was the lowest,and the quantitative estimation values of MMP-2 and MMP-9 were(0.89)±(0.02) and(0.86)±(0.01),respectively.However,the quantitative estimation values of MMP-2 and MMP-9 in the conditioned medium of C4-2B were(96.32)±(4.36) and(33.89)±(1.84),respectively.Compared with LNCaP,the activity of type IV collagenase in C4-2B was much higher(P<(0.01)).Conclusion: Compared with LNCaP,the higher production capability of type IV collagenase in C4-2B may possibly contribute to its invasive and metastatic potentials.The expression of type IV collagenase of human prostate carcinoma cells is closely correlated to the metastatic and invasive potential.