小鼠不同肿瘤模型对放化疗敏感性的研究

目的 建立人食管癌及小鼠不同移植瘤模型，比较放、化疗对小鼠的不同肿瘤模型的抑制作用。 方法 动物模型建立24 h后，将不同肿瘤细胞（淋巴瘤、肉瘤S180、肝癌H22、白血病L1210）的荷瘤IRM-2近交系小鼠随机分为对照组、照射组、环磷酰胺组，每组10只。环磷酰胺组于接种隔日腹腔注射环磷酰胺（25 mg/kg）1次，0.2 ml/只，共4次。照射组于接种后第4天进行全身1 Gy照射，每日1次，连续5 d。将食管癌EC901荷瘤裸鼠随机分为对照组、照射组、5-氟尿嘧啶组，每组5只。照射组于接种后第4天和第8天对肿瘤局部进行2 Gy照射，5-氟尿嘧啶组于接种后隔日腹腔注射5-氟尿嘧啶（25 mg/kg）1次，0.2 ml/只，共4次。所有小鼠于第12天处死，解剖瘤块称重，计算抑瘤率。 结果 裸鼠及IRM-2近交系小鼠皮下移植瘤模型的成瘤率均为100%，放疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210荷瘤小鼠的肿瘤有显著的抑制作用，抑瘤率分别为34.57%、32.69%、31.31%和18.32%，与对照组比较差异有统计学意义（t=4.130、3.222、3.581和2.713，P分别为 < 0.01、 < 0.01、 < 0.01、 < 0.05）。放疗对食管癌EC901的抑瘤率为22.99%，但与对照组比较差异无统计学意义（t=1.235，P > 0.05）。化疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210、食管癌EC901荷瘤小鼠的抑瘤率分别为74.47%、72.59%、69.12%、77.53%和56.32%，与对照组比较差异有统计学意义（t=12.694、12.208、7.223、11.964和5.266，P均 < 0.01）。 结论 放、化疗对小鼠的不同肿瘤模型均有抑制作用。由于小鼠的品系和瘤源不同，对放、化疗的敏感性也存在差异。

The radiation sensitivities of different mice tumor model to radiotherapy and chemotherapy

Objective To set up the models of human esophageal cancer in nude mice and the models of different xenograft tumors in IRM-2 mice, observe the effects of chemotherapy and radiotherapy on the tumors. Methods Twenty-four hours after setting up the models, all the mice carrying tumors including lymphoma, hepatic carcinoma H22, leukemia L1210 and sarcoma S180 were randomly divided into 3 groups： control group, radiotherapy group and cyclophosphamidum group, 10 mice per group. Mice in cyclophos- phamidum group were injected cyclophosphamidum （25 mg/kg） by intraperitoneal 4 times every other day; mice in radiotherapy group were given total body irradiation at the fourth day and last for 5 days. Mice with human esophageal cancer were randomly divided into 3 groups： control group, radiotherapy group and 5-fluo- rouracil group, 5 mice per group. Mice in radiotherapy group were given local body irradiation at the fourth day and the eighth day. Mice in 5-fluorouraeil group were injected 5-fluorouraeil （25 mg/kg） by intraperi- toneal 4 times every other day. All the mice were killed at the twelfth day and the rates of tumor inhibition were calculated. Results The tumor inhibitory rates of IRM-2 mice in radiotherapy group were 34.57% （lymphoma）, 32.69%（sarcoma S 180）, 31.3 l%（hepatic carcinoma H22） and 18.32%（leukemia L1210） respec- tively, and there were significant difference compared with control group（t=4.130, 3.222, 3.581 and 2.713, P〈0.OI, 〈0.01, 〈0.01 and 〈0.05）. The tumor inhibitory rates of nude mice with human esophageal cancer in radiotherapy group was 22.99%, but there was no significant difference compared with control group（t= 1.235, P〉0.05）. The tumor inhibitory rates in radiotherapy group were 74.47%（lymphoma）, 72.59%（sarcoma S180）, 69.12%（hepatic carcinoma H22）, 77.53%（leukemia L1210） and 56.32%（human esophageal cancer）, and there were significant difference compared with control group（t =12.694, 12.208, 7.223, 11.964 and 5.266, all P〈0.01）. Conclusions There were inhibitory effects of chemotherapy and radiotherapy on all types of tumors. However, the efficiency depends on the mouse strains and the type of tumor.