Palomo和改良Palomo曲张精索内静脉结扎术对大鼠同侧睾丸的影响

目的 评估PMomo曲张精索内静脉结扎术(PV)和改良Palomo曲张精索内静脉结扎术(MPV)对大鼠同侧睾丸的影响.方法 检测实验性左侧精索静脉曲张(ELV)组(9只)、PV组(10只)、MPV组(8只)、和对照组(8只)大鼠左侧睾丸的Johnsen's评分、生精小管的超微结构、和生殖细胞凋亡指数(AJ).结果 对照组Johnsen's评分(9.46±0.52)显著高于ELV组(7.72±0.31,P＜0.05)和PV组(2.86±0.31,P＜0.01),与MPV组(9.42±0.63)比较差异无统计学意义(P＞0.05);对照组AI(2.21±1.15)显著低于ELV组(5.32±1.23,P＜0.05)和PV组(9.26±1.98,P＜0.01),与MPV组(2.32±1.18)比较差异无统计学意义(P＞0.05);ELV和PV组生精小管的超微结构明显异常.结论 MPV可以修复ELV所导致的同侧睾丸的损伤,PV则使损伤进一步加重.

The effects of Palomo and modified Palomo varicocelectomy on the ipsilateral testes in rats

Objective To investigate the effects of Palomo varicocelectomy (PV) and modified Palomo varicocelectomy (MPV) on the ipsilateral testes in rats. Methods The Johnsen's score was re-corded, ultrastructure of seminiferous tubules observed, and germ cell apoptotic index (AI) detected in the left testes in the experimental left varicocele (ELV) group (n = 9), PV group (n = 10), MPV group (n =8) ,and control group (n = 8). Results Johnsen's score in control group (9.46±0.52) was significant-ly higher than that in ELV group (7.72±0.31,P＜0.05) and PV group (2.86±0. 31, P＜0.01), but there was no statistically significant difference between control group and MPV group (9.42±0.63, P＞0.05).Germ cell AI in control group (2.21±1.15) was significantly lower than that in ELV group(5.32±1.23, P＜0.05) and PV group (9.26±1.98, P＜0.01), but had no statistically significant difference from that in MPV group (2.21±1.15 ,P＞0.05). Ultrastructural abnormalities of seminiferous tubules were observed in PV and ELV groups. Conclusion The MPV can repair the varicocole-induced lesion on the ipsilateral testes,but the PV can aggravate the injury.