醛糖还原酶基因5’端(AC)n多态性对2型糖尿病患者红细胞醛糖还原酶活性的影响

目的　探讨醛糖还原酶 (AR)基因 5’端 (AC) n 的多态性对 2型糖尿病 (DM )红细胞AR活性的影响。方法　 16 3例 2型DM分为无微血管病变 (NDC)组 (6 6例 )和微血管病变 (DMAP)组(97例 ) ,正常对照 (CON)组 42例 ;另按AR基因 5’端 (AC) n 的等位基因类型分为DM携带Z 2等位基因 (DZ 2 )组 (5 4例 )、DM携带Z - 2等位基因 (DZ - 2 )组 (35例 )、DM同时携带Z 2和Z - 2等位基因 (Z 2 /Z - 2 )组 (18例 )、DM不携带Z 2和Z - 2等位基因 (X/X)组 (5 6例 )、对照者携带Z 2等位基因 (NZ 2 )组 (2 1例 )和对照者携带Z - 2等位基因 (NZ - 2 )组 (7例 )。用改良Sriratava法测定AR活性并比较其在各组间的差异。结果　DMAP组、NDC组和CON组间的AR活性 (ARA)差异有显著性 ,DMAP组最高 ,NDC组次之 ,CON组最低 (P <0 .0 0 1)。DM组携带Z - 2和Z 2等位基因各亚组中 ,DZ - 2组ARA最高 ,Z - 2 /Z 2和X/X组居中 ,DZ 2组最低 ,差异有显著性统计学意义(P <0 .0 0 1)。DZ - 2和NZ - 2组的ARA分别高于DZ 2和NZ 2组 ,DZ - 2和DZ 2组的ARA分别高于NZ - 2和NZ 2组 (P均 <0 .0 0 1)。结论　AR的激活对DMAP的发生和发展起重要作用。Z - 2等位基因可能是AR的激活因子 ,Z 2等位基因则为其抑制因子。

The effect of the polymorphism of (AC)_n in the 5'-end of the aldose reductase gene on the erythrocyte aldose reductase activity in the patients with type 2 diabetes mellitus

Objective To study the effect of the polymorphism of (AC) n of aldose reductase (AR) gene on the erythrocyte AR activity in type 2 diabetes mellitus (DM). Methods One hundred and sixty three cases of type 2 DM and forty two controls were included in this study. The subjects were devided into DM without diabetic microangiopathy (DMAP) (NDC) (66 cases), DMAP groups (97 cases), and normal controls (CON) (42 cases); further, according to the type of allele, the subjects were devided into DM with Z 2 (DZ 2) (54 cases), DM with Z-2 (DZ-2) (35 cases), DM with both Z 2 and Z-2 (Z 2/Z-2) (18 cases), DM with neither Z 2 nor Z-2 (X/X) (56 cases), non DM with Z 2 (NZ 2) group (21 cases) and non DM with Z-2 (NZ-2) groups (7 cases). AR activity (ARA) was measured by modified Sriratava method. The differences of ARA were compared among these groups. Results ARA in DMAP group was significantly higher than that in NDC group, ARA in NDC group was significantly higher than that in CON group. Among DZ-2, DZ 2, Z 2/Z-2 and X/X groups, ARA was the highest in DZ-2 group and the lowest in DZ 2 group. ARAs in DZ-2 and NZ-2 groups were significantly higher than those in DZ 2 and NZ 2 groups respectively. In DZ-2 and DZ 2 groups ARA was significantly higher than that in NZ-2 and NZ 2 groups respectively. Conclusion The activation of AR plays an important role in the development of DMAP. Z-2 allele may be an activator and Z 2 allele may be an inhititor of AR.