血管紧张素系统基因多态性与早发冠心病的相关性研究

目的探讨血管紧张素转换酶（ACE）和血管紧张素Ⅱ1型受体（AT1R）基因多态性与早发冠心病（CAD）的关系。方法用PCR法检测41例早发CAD和64例迟发CAD患者体细胞ACE基因插入／缺失（I／D）多态性和AT1R-A1166C基因多态性，并与50例健康者对照。结果105例CAD患者ACE的DD基因频率分布与对照组比较差异有显著性（P〈0．05）；早发和迟发CAD患者与对照组比较，D等位基因和DD基因频率显著高于对照组（P〈0．05）。早发CAD组AT1R的CC基因型和C等位基因频率与对照组相比差异有显著性（P=0．036，P=0．008）。联合基因多态分析，早发CAD合并ACE-DD＋AT1 R—CC基因型频率显著高于对照组（P=0．036）；迟发CAD合并ACE-DD＋AT1 R—CC基因型频率与对照组比较差异无显著性（P=0．206）。结论早发CAD组与ACE的DD基因频率和ATIR的A1166C基因频率分布相关，ACE-DD基因频率与AT1R—CC基因频率在早发CAD的发病中具有协同作用。

Relationship between angiotensin system gene polymorphisms and premature coronary artery disease

Objective To investigate the association between angiotensin converting enzyme(ACE)and angiotensin II receptor I polymorphisms(AT1R) with the premature coronary artery disease(CAD).Methods By using PCR analysis method,insertion/deletion polymorphisms of angiotensin-converting enzyme(ACE-I/D) gene and A1166C polymorphisms of angiotensin II receptor I(AT1R-A1166C)gene were analyzed in 41 patients in premature CAD and 64 patients in mature CAD group as well as 50 healthy subjects in control group.Results ACE-DD genotype was more frequent in premature and mature CAD groups than those of control group(P<0.05);ACE-DD genotype and D alleles were more frequent in premature and mature CAD groups than those of control group(P<0.05);AT_1R-CC genotype and C alleles were more frequent in premature CAD groups than those of control group( P=0.036 ,P=0.008);The combining analysis indicated that ACE-DD and AT1R-CC genotype frequency was significantly higher in premature CAD group than those in control group(P=0.036);ACE-DD and AT1R-CC frequency was not significantly different in mature CAD group than those of control group(P=0.206).Conclusion The distribution of ACE-DD genotype and AT1R-A1166C genotype are associated with premature CAD,and the frequency of ACE-DD and AT_1R-CC genotype has a synergistic effect on the risk of premature CAD.