柏氏疟原虫红内期体外培养的初步研究

按烛缸法对柏氏疟原虫(Plasmodium berghei)红内期进行了体外培养的初步研究。先后5次,每次连续培养7天。除培养的第2天,其原虫率稍有增长外,随后便逐日下降;与此同时,培养的第4天,环状体有增多外,其后也逐日减少,而未成熟裂殖体及成熟裂殖体相对地逐日增加。培养结果提示有1～2个周期的裂体增殖。另将第7天的培养物无菌注入健康正常小白鼠腹腔内,7天后血检,原虫率明显上升(>5％),各红内期形态均可见到,还偶见到配子体,鼠间继续人工传代,原虫活力不减,实验鼠均于1周左右自然致死,反映原虫经体外培养7天后对宿主仍具有很强的毒力。

Preliminary Study on the Cultivation in Vitro of Erythrocytic Stage of Plasmodium Berghei

According to Jensen and Trager's candle jar method, five continuous cultivation, in vitro experiments were conducted with Plasmodium berghei. In all of the experiments, the parasites could be seen to go through their erythrocytic cycle, but only a few of merozoites were able to enter the other red blood cells to complete their intra-erythrocytic cycle. These experiments showed 1～2 schizogony of the parasites ia vitro. Parasites in the fractional blood of the 7th day's culture (parasitemia 0.1％) were determined by aseptical intraperitoneal injection into the clean mice. The blood parasitemia was mo-nitored after maintaing 7 days. The results were as fellows: (1) The parasitemia went upgrade over 5％. (2) In the blood slide, a few of male and female gametocytes could be seen. (3) The experimental mice died about 7 days after artificial infections. what has been said above, shows that though the erythrocytic stages of plasmodium berghei had been cultivated in vitro for continuous 7 days, the parasites still maintained infectivity and pathogenicity to the host.