Cloning of human hybridoma, myeloma and lymphoma cell lines using enriched human monocytes as feeder layer |
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Authors: | T Brodin L Olsson H O Sj?gren |
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Abstract: | Human monocytes were prepared from peripheral blood by buoyant density centrifugation and subsequent absorption-elution on a column of gelatin beads. The eluted fraction containing 60-80% monocytes was used as feeder layer in cloning of the human lymphoma line RH-L4, the human myeloma line SKO-007, and a human hybridoma cell derived from the latter line. Cloning efficiencies were high in both liquid and semisolid media with all 3 cell lines tested. Feeder monocytes could also be successfully used after having been stored in liquid nitrogen. |
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