葛根总黄酮逆转链脲佐菌素诱导糖尿病小鼠胰腺损伤的研究

目的:研究葛根总黄酮逆转链脲佐菌素(STZ)诱导糖尿病小鼠胰腺损伤.方法:STZ(150 mg·kg-1)ip复制糖尿病小鼠模型,随机分成5组:模型组、二甲双胍阳性组及葛根总黄酮低、中、高剂量组.ig给予葛根总黄酮80,160,240 mg·kg-1,阳性组给予二甲双胍80 mg·kg-1,持续给药14 d.采用葡萄糖氧化酶法检测空腹血糖(FBG)水平.放射免疫法检测空腹血清胰岛素(FIns)及胰岛素抵抗(IR)水平.ELISA法检测胰腺组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、活性氧(ROS)活性和丙二醛(MDA)含量.HE染色观察胰腺细胞病理学的变化.Western blot法检测胰脏血红素加氧酶1 (HO-1)的表达.结果:与模型组比较,葛根总黄酮有效降低糖尿病小鼠的血糖水平(P＜0.01).恢复胰岛素水平,改善IR(P ＜0.01).增加胰腺GSH-Px、SOD活性,同时降低ROS,MDA水平(P＜0.01).逆转STZ致糖尿病小鼠胰岛β细胞损伤.有效下调胰腺HO-1蛋白表达(P＜0.01).结论:葛根总黄酮对STZ致糖尿病小鼠胰腺损伤有保护作用,机制可能与改善血糖代谢和抗氧化应激有关.

Experimental Study of Reversion of Puerariae Radix Lobatae Flavonoids on Pancreas Injury Induced by Streptozotocin in Mice

Objective: To investigate the experimental study of reversion of Puerariae Radix Lobatae flavonoids on pancreas injury induced by streptozotocin in mice. Method: Being established diabetic mice by intraperitoneal injection of streptozotocin (150 mg·kg^-1) firstly, and then the mice were randomly divided into 5 groups: model group, dimethyldiguanide group (80 mg·kg^-1), low-, moderate-and high-dose groups of Puerariae Radix Lobatae flavonoids (80, 160, 240 mg·kg^-1). The drugs were intragastrically given to mice daily for 14 days. The level of fasting blood glucose (FBG) was tested by glucose oxidase method. The levels of fasting serum insulin (FIns) and insulin resistance (IR) were measured via radioimmunoassay (RIA).The activities of glutathione-peroxidase (GSH-Px), superoxide dismutase (SOD) in pancreas tissue, and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were using ELISA test. The pathologic changes of pancreatic cells were observed by HE staining. The expression of heme oxygenase-1 (HO-1) in pancreas was through Western blot analysis. Result: Compared to model control group, Puerariae Radix Lobatae flavonoids effectively lowered the blood-glucose level in diabetic mice (P<0.01). The insulin level in serum was restored, and IR condition was improved (P<0.01). The activities of GSH-Px, SOD in pancreas tissue were elevated, while the content of ROS, MDA was lessened (P<0.01). And the islet beta cells injure was reversed. The HO-1 expression was down-regulated in pancreatic tissue (P<0.05). Conclusion: The findings demonstrate that Puerariae Radix Lobatae flavonoids mediate protection against pancreas injury induced by STZ in mice, though the mechanisms involved in amelioration of metabolism in blood glucose and suppression of oxidative stress.