补阳还五汤和星蒌承气汤促进脑缺血大鼠神经修复和对肝肾功能的保护研究

目的:观察补阳还五汤和星蒌承气汤对脑缺血大鼠神经功能修复的作用,并从对谷草转氨酶(AST)、谷丙转氨酶(ALT)及肌酐(Cr)、尿素氮(BUN)的影响观察其对肝肾功能的保护作用.方法:SD大鼠120只随机分为假手术组、模型组、尼莫地平组、星蒌承气汤和补阳还五汤组；线栓法制备大脑中动脉阻塞模型；大鼠灌胃用药14 d,分别于14,28 d进行神经功能评测,检测大鼠肝肾功能变化.结果:与假手术组相比,模型组大鼠14,28 d神经功能评分均明显降低,AST,ALT,Cr,BUN明显升高.与模型组相比,各药物组大鼠14、28 d神经功能评分均明显升高,28 d AST,ALT水平降低；星蒌承气汤降低14 dAST水平作用明显,补阳还五汤降低28 d Cr作用明显.与尼莫地平及星蒌承气汤组比较,28 d补阳还五汤组神经功能评分明显升高,Cr显著降低.与14 d组比较,各药物组大鼠28 d神经功能评分均明显升高；28 d补阳还五汤组AST,ALT,Cr降低明显.结论:脑缺血后大鼠神经功能的自然修复缓慢,补阳还五汤和星萎承气汤对脑缺血远期的神经修复具有促进作用,以补阳还五汤的作用尤为明显；脑缺血可引起肝肾功能受损,星萎承气汤和补阳还五汤在缺血后不同时期保护肝肾功能的作用存在差异,其中以补阳还五汤保护肝肾功能的作用尤为全面而显著.

Effects of Buyang Huanwu Decoction and Xinglou Chengqi Decoction on Nerves Functional Rehabilitation and Protection of Liver and Kidney Function in Rats with Cerebral Ischemia

Objective: To observe the effects of Buyang Huanwu decoction(BYHWD) and Xinglou Chengqi decoction(XLCQD) on the nerves functional rehabilitation and its protective effect on aminotransferase, creatinine(Cr) and blood urea nitrogen(BUN) were evaluated for Liver and kidney function in rats with cerebral ischemia. Method: Rats were divided randomly into sham,model,nimodipine,BYHWD and XLCQD groups. Rat focal cerebral ischemia model was established by middle cerebral artery occlusion (MCAO) with nylon thread.Rats were administrated with corresponding drugs by gavage before establishing model. After operation of cerebral ischemia, rats were given the corresponding drugs once a day for 14 days.Rats neurological score (NS) was evaluated and blood was taken out at 14 d and 28 d after operation,and then the rat's liver and kidney function were determined. Result: In each model group,rats NS decreased,aspartate aminotransferase(AST),alanine aminotransferase(ALT),Cr,BUN increased. In comparison with that of rats in each model group, NS of rats in each treatment group raised, and AST,ALT,Cr,BUN reduced obviously except 14 d in BYHWD group. In comparison with that in nimodipine and XLCQD group,the NS increased in 28 d BYHWD group,Cr in 28 d BYHWD group reduced Significantly. NS of rats obviously ascended in each treatment group and AST,ALT decreased in 28 d BYHWD group. Conclusion: After cerebral ischemia the neurological function of rats restorated slowly. It is indicated that the treatment of XLCQD and BYHWD can Promote the recovery of neurological function injuried by cerebral ischemia and BYHWD has the more significant roles in the recovery of neurological function. Cerebral ischemia can lead to liver and kidney function damage, BYHWD and XLCQD play a different role in different time after cerebral ischemia, and the role of BYHWD is more comprehensive and significant.