吗啡依赖对小鼠海马内一氧化氮合酶阳性神经元的影响

目的·· :探讨吗啡依赖对小鼠海马不同亚区一氧化氮合酶(NOS)活性的影响。方法·· :以剂量递增法皮下注射吗啡建立吗啡依赖小鼠模型 ,采用还原型辅酶Ⅱ -黄递酶(NADPH -d)组织化学法显示吗啡依赖组、纳洛酮催促戒断组和正常对照组小鼠海马CA1区、CA3区和齿状回NOS阳性神经元。结果·· :与对照组相比 ,吗啡依赖组和纳洛酮催促戒断组海马CA1区和齿状回NOS阳性神经元的数目均明显减少 (P<0.01) ,纳洛酮催促戒断组更为明显 ,而在CA3区无明显改变。结论·· :吗啡依赖和纳洛酮催促戒断小鼠海马CA1区和齿状回NOS活性降低 ,提示NO合成的能力下降 ,戒断期下降更明显。这些变化可能是吗啡依赖造成学习记忆功能下降的原因之一。

IMPACT OF MORPHINE DEPENDENCE ON NOS POSITIVE NEURONS IN MICE HIPPOCAMPUS

Objective: To study the impact of morphine dependence on the activity of NOS in subareas of mice hippocampus.Methods: Mice were given (sc) gradually increasing doses of morphine to form morphine dependent model. NADPH-diaphorase with a histochemical method was used to study the distribution areas of NOS positive neurons in CA1, dentate gyrus and CA3 of hippocampus of morphine dependent group, naloxone-precipitated withdrawal group in morphine dependent mice and control group. Results: Compared with control group , the number of positive cells decreased obviously in CA1 and dentate gyrus of both groups in morphine dependent mice, especially the later. Conclusion: The activity of NOS in CA1 and dentate gyrus of morphine dependent and naloxone-precipitated withdrawal groups has decreased, especially the later, which suggests that the synthesis of NO has decreased. Those changes may be some of the reasons causing learning and memory deficits.