Characterization and Genomic Analysis of Novel Vibrio parahaemolyticus Phage vB_VpaP_DE10 |
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| Authors: | Yuanming Ye Hanfang Chen Qiaolan Huang Shixuan Huang Jiaxin He Jumei Zhang Qingping Wu Xueling Li Wenfeng Hu Meiyan Yang |
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| Affiliation: | 1.College of Agriculture, College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China; (Y.Y.); (H.C.); (Q.H.); (S.H.); (J.H.); (X.L.);2.Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; (J.Z.); (Q.W.) |
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| Abstract: | In the present study, a novel lytic Vibrio parahaemolyticus phage, vB_VpaP_DE10, was isolated from sewage samples collected in Guangzhou city, China. Transmission electron microscopy revealed that phage vB_VpaP_DE10 has an icosahedral head (52.4 ± 2.5 nm) and a short non-contracted tail (21.9 ± 1.0 nm). Phage vB_VpaP_DE10 lysed approximately 31% (8/26) of the antibiotic-resistant V. parahaemolyticus strains tested. A one-step growth curve showed that phage vB_VpaP_DE10 has a relatively long latency time of 25 min and a burst size of ~19 PFU per cell. The genome of phage vB_VpaP_DE10 is a 42,871-bp-long dsDNA molecule with a G + C content of 49.19% and is predicted to contain 46 open reading frames, 26 of which are predicted to be related to functions such as phage structure, packaging, host lysis, and DNA metabolism. Sequence comparisons suggested that vB_VpaP_DE10 is a member of the genus Maculvirus within the family Autographiviridae. Morphological and genomic analysis indicated that vB_VpaP_DE10 is a novel V. parahaemolyticus phage. |
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| Keywords: | Vibrio parahaemolyticus phage Autographiviridae genome analysis |
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