膈下迷走神经切断术抑制腹腔给予LPS诱导的延髓内脏带内Fos表达

目的　研究迷走神经在免疫系统至延髓内脏带 (MVZ)通路中的可能性。　方法　对雄性 SD大鼠行膈下双侧迷走神经干切断术或假切断手术 ,存活 4周后再经腹膜腔注入免疫刺激剂脂多糖 (L PS)或无菌生理盐水(NS) ,3h后处死 ,用免疫组织化学 ABC法对 MVZ进行 Fos染色。　结果　假手术组大鼠腹腔给予 L PS后 ,MVZ可发现大量 Fos表达 ;膈下迷走神经切断术组大鼠腹腔给予 L PS后 ,MVZ内的 Fos表达量则明显减少。　结论　迷走神经参与外周免疫信息向中枢的传递 ,外周免疫信息可经迷走神经传至 MVZ,MVZ是“免疫 -脑通讯”的中继站 ,机体内存在“迷走神经→ MVZ”神经免疫调节通路。

SUBDIAPHRAGAMATIC VAGOTOMY INHIBITS Fos EXPRESSION IN THE MEDULLARY VISCERAL ZONE AFTER INTRAPERITONEAL ADIMINISTRATION OF LIPOPOLYSACCHARIDE

Objective To test the possibility that the vagus nerve is involved in the communication between the immune system and the medullary visceral zone(MVZ). Methods Male Sprague|Dawley rats were received either subdiaphragmatic vagotomy or sham operation.Four weeks later,rats were challenged with pyrogen|free saline or lippolysaccharide(LPS) by intraperitoneal route,and sacrificed 3hrs later.Medulla sections were processed for Fos immunoreactivity using ABC method. Results Intraperitoneal LPS caused robust Fos expression within the MVZ in the sham surgery groups and this response in the MVZ was inhibted in the vagotomized rats. Conclusions This study suggested that the abdominal vagus nerve consituted an important immune signaling pathway from immune system to the MVZ,and MVZ was a relay station in the immune|to|brain communication.MVZ might play a prominent role in the neuroimmunomodulation via vagus|to|MVZ neuroimmunomodulatory pathway.