| 种间转移扩增法筛选长爪沙鼠微卫星位点 |
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| 引用本文： | 谭元卿,李薇,杜小燕,路静,吴艳花,王超,陈振文.种间转移扩增法筛选长爪沙鼠微卫星位点[J].实验动物与比较医学,2011,19(1). |
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| 作者姓名： | 谭元卿 李薇 杜小燕 路静 吴艳花 王超 陈振文 |
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| 作者单位： | 首都医科大学基础医学院实验动物学系,北京,100069 |
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| 基金项目： | 国家自然科学基金项目(No.30570261); 国家科技支撑计划课题(No.2009BAI83B02); 北京市教委重点项目(No.KZ200910025002) |
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| 摘 要： | 目的筛选长爪沙鼠新的微卫星位点,为长爪沙鼠遗传分析提供遗传标记物。方法从GenBank中随机选取小鼠微卫星位点引物536对,用这些引物对长爪沙鼠基因组DNA扩增,将阳性目的条带进行序列分析,找出符合微卫星序列特征的短串联重复序列。结果 536对小鼠微卫星引物在长爪沙鼠基因组中扩增出了313个阳性条带,经序列分析,确定130个长爪沙鼠微卫星位点;其中完美型位点占80.77%(105/130),不完美型位点占19.23%(25/130),与小鼠同源性为24.3%(130/536)。将筛选出的微卫星位点在GenBank中注册,注册号从GU562694到GU562823。结论小鼠和沙鼠的微卫星位点具有较高的同源性,用小鼠的微卫星位点引物直接扩增长爪沙鼠基因组DNA可有效地筛选出长爪沙鼠微卫星位点。
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| 关 键 词： | 小鼠 长爪沙鼠 微卫星 种间转移扩增 |
Screening of novel microsatellite DNA markers in Mongolian gerbils by cross-amplification |
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| TAN Yuan-qing,LI Wei,DU Xiao-yan,LU Jing,WU Yan-hu,WANG Chao,CHEN Zhen-wen.Screening of novel microsatellite DNA markers in Mongolian gerbils by cross-amplification[J].Laboratory Animal and Comparative Medicine,2011,19(1). |
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| Authors: | TAN Yuan-qing LI Wei DU Xiao-yan LU Jing WU Yan-hu WANG Chao CHEN Zhen-wen |
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| Institution: | TAN Yuan-qing,LI Wei,DU Xiao-yan,LU Jing,WU Yan-hua,WANG Chao,CHEN Zhen-wen(Department of Laboratory Animal Sciences,Capital Medical University,Beijing 100069,China) |
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| Abstract: | Objective To screen new microsatellite loci in Mongolian gerbils to develop genetic markers for genetic analysis.Methods 536 mouse loci were randomly selected from GenBank and amplified in Mongolian gerbils genetic DNA.The positive PCR products were sequenced and confirmed as simple sequence repeat(SSR) loci.Result Of these 536 mouse markers,313(58.39%) were discretely amplified from the laboratory gerbils.Of these 313 sequenced markers,130 were confirmed as SSR loci in the gerbil,in which 105 loci(80.77%) ... |
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| Keywords: | Mouse Mongolian gerbil Microsatellite Cross-amplification |
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