Circular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation,migration, invasion,and apoptosis

Background

Recent studies have found circular RNAs (circRNAs) involved in the biological process of cancers. However, little is known about their functional roles in glioblastoma.

Methods

Human circRNA microarray analysis was performed to screen the expression profile of circRNAs in IDH1 wild‐type glioblastoma tissue. The expression of hsa_circ_0008344 in glioblastoma and normal brain samples was quantified by qRT‐PCR. Functional experiments were performed to investigate the biological functions of hsa_circ_0008344, including MTT assay, colony formation assay, transwell assay, and cell apoptosis assay.

Results

CircRNA microarray revealed a total of 417 abnormally expressed circRNAs (>1.5‐fold, P < .05) in glioblastoma tissue compared with the adjacent normal brain. Hsa_circ_0008344, among the top differentially expressed circRNAs, was significantly upregulated in IDH1 wild‐type glioblastoma. Further in vitro studies showed that knockdown of hsa_circ_0008344 suppressed glioblastoma cell proliferation, colony formation, migration, and invasion, but increased cell apoptotic rate.

Conclusions

Hsa_circ_0008344 is upregulated in glioblastoma and may contribute to the progression of this malignancy.