| 表现神经症状的SIVmac251感染猴大脑基底节病毒gp120序列变异分析 |
| |
| 引用本文： | 刘克剑,丛喆,金光,王卫,陈霆,蒋虹,魏强. 表现神经症状的SIVmac251感染猴大脑基底节病毒gp120序列变异分析[J]. 实验动物与比较医学, 2012, 0(2): 37-42 |
| |
| 作者姓名： | 刘克剑 丛喆 金光 王卫 陈霆 蒋虹 魏强 |
| |
| 作者单位： | 中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021;中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 |
| |
| 摘 要： | 目的研究猴免疫缺陷病毒SIVmac251在中国恒河猴感染传代过程中产生的可能的神经侵袭性和神经嗜性及其分子机制。方法从静脉感染SIVmac251-155p6N的8只实验猴中出现严重神经症状的1只猴中,监测病毒及免疫指标变化,观察临床症状、猴脑组织病变,单拷贝PCR扩增病毒gp120序列并分析变异及糖基化位点变化情况。结果感染猴晚期出现明显艾滋病脑病症状,病理切片显示脑组织出现多核巨细胞及神经元变性、坏死。脑基底节分离出单一序列病毒,其氨基酸序列与血浆病毒及感染毒株SIVmac251-155p6序列差异主要位于Gp120的V1和V4区,并且在C1区66位出现一个糖基化位点缺失。结论SIVmac251在猴体长期传代过程中表现出神经嗜性毒株的特征,对AIDS脑病研究具有重要意义。
|
| 关 键 词： | 艾滋病脑病 SIVmac251 gp120 变异 神经嗜性 神经侵袭性 糖基化位点 |
Characterization of the SIVmac251 in a Chinese-origin rhesus macaque showing severe neurological symptoms and sequence variation of Gp120 |
| |
| LIU Ke-jian,CONG Zhe,JIN Guang,WANG Wei,CHEN Ting,JIANG Hong and WEI Qiang. Characterization of the SIVmac251 in a Chinese-origin rhesus macaque showing severe neurological symptoms and sequence variation of Gp120[J]. Laboratory Animal and Comparative Medicine, 2012, 0(2): 37-42 |
| |
| Authors: | LIU Ke-jian CONG Zhe JIN Guang WANG Wei CHEN Ting JIANG Hong WEI Qiang |
| |
| Affiliation: | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China;Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China |
| |
| Abstract: | |
| |
| Keywords: | NeuroAIDS SIVmac251 Gp120 Sequence variation Neurotropic Neuroinvasive N-glycosites Rhesus macaque |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
|
点击此处可从《实验动物与比较医学》下载全文 |
|
