中药白花前胡提取工艺的优化

目的为合理提取白花前胡中降肺动脉高压的活性成分提供实验依据。方法采用正交试验法,考察提取溶媒、提取次数、提取时间对提取效率的影响;采用HPLC法,以8-甲氧基补骨脂素含量为指标测定提取物含量。结果白花前胡的最佳提取工艺为用质量分数80%的乙醇提取3次,提取时间分别为1.0、0.5、0.5 h,3次所用溶媒量分别为药材质量的8、6、4倍;按照筛选出的最佳工艺条件进行的3次重复性实验,测得浸膏中8-甲氧基补骨脂素含量质量分数为0.094%,折合1 g生药中含量为0.1712 mg,与预测值基本一致。结论所确定的最佳提取工艺可合理提取白花前胡中的活性成分。

Optimization of extracting process of roots of Peucedanum praeruptorum Dunn

Abstract: Objective To optimize the conditions for the extraction of roots of Peucedanum praeruptorum Dunn. Methods The conditions for the extraction were studied by the orthogonal design with the weight of extracts and the content of 8-methoxypsoralen as indexes. Results The optimum conditions for the extraction of Peucedanum praeruptorum Dunn was as follow: 80 % alcohol was used as solvent, and 8 times the amount of alcohol was added, the mixture was kept refluxing for 1.0 h for the first time; then 6 times the amount of alcohol was added and the mixture was kept refluxing for 0.5 h for the second time; Four times the amount of alcohol for 0.5 h for the last time. The results of confirmatory test showed that the content of 8-methoxypsoralen was 0.094 % in extracts and 0.171 2 mg in 1 g of materials, which were consistent with the predictive values. Conclusions The optimized method can be applied for the extraction of active components in roots of Peucedanum praeruptorum Dunn.