| LC-MS/MS法同时测定人尿液中5-氟尿嘧啶和吉美嘧啶浓度及其尿药排泄研究 |
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| 引用本文： | 刘荷英,储 妍,于 勇,丁 黎. LC-MS/MS法同时测定人尿液中5-氟尿嘧啶和吉美嘧啶浓度及其尿药排泄研究[J]. 药学与临床研究, 2010, 18(3): 256-259 |
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| 作者姓名： | 刘荷英 储 妍 于 勇 丁 黎 |
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| 作者单位： | 1. 中国药科大学药物分析教研室,南京,210009
2. 中国药科大学药物分析教研室,南京,210009;中国药科大学药物质量与安全预警教育部重点实验室,南京,210009 |
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| 摘 要： | 目的:建立5-氟尿嘧啶和吉美嘧啶尿药浓度的LC-MS/MS同时测定方法,研究胃癌患者口服替吉奥胶囊后其活性代谢物5-氟尿嘧啶和增效剂吉美嘧啶的人体尿药排泄特征.方法:尿液样品酸化后用异丙醇-乙酸乙酯(15:85)提取,采用Hedera ODS-2色谱柱分离,流动相为甲醇-0.01%的乙酸水溶液(35:65);质谱采用电喷雾离子源和负离子多反应离子监测方式.6名胃癌患者单次口服替吉奥胶囊60mg后,测定尿药浓度,评价其尿药排泄特征.结果:5-氟尿嘧啶和吉美嘧啶的最低定量下限分别为0.15μg·mL-1和0.10μg·mL-1;高、中、低浓度的日间与日内精密度均小于15%.6名胃癌患者单次口服替吉奥胶囊后尿液中5-氟尿嘧啶和吉美嘧啶的平均累积排泄率分别为4.9%和18.1%.结论:建立的LC-MS/MS分析方法适用于尿药排泄研究.
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| 关 键 词： | 5-氟尿嘧啶 吉美嘧啶 替吉奥 尿药排泄 |
| 收稿时间： | 2010-03-29 |
| 修稿时间： | 2010-04-22 |
Simultaneous Determination of 5-fluorouracil and 5-chloro-2, 4-dihydroxypyridine by LC-MS/MS in Human Urine and Study on Their Urinary Excretion |
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| LIU He-ying,CHU Yan,YU Yong and DING Li. Simultaneous Determination of 5-fluorouracil and 5-chloro-2, 4-dihydroxypyridine by LC-MS/MS in Human Urine and Study on Their Urinary Excretion[J]. Pharmacertical and Clinical Research, 2010, 18(3): 256-259 |
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| Authors: | LIU He-ying CHU Yan YU Yong DING Li |
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| Affiliation: | Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009;Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009;Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009;Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China |
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| Abstract: | Objective: To develop an LC-MS/MS method for the simultaneous determination of 5-fluorouracil (a major active metabolite of tegafur) and 5-chloro-2, 4-dihydroxypyridine (a synergistic agent) in urine samples, and to investigate their urinary excretion cha |
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| Keywords: | LC-MS/MS |
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