| 人胚胎肾间质成纤维细胞培养及其鉴定 |
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| 引用本文： | 潘晓勤,黄文彦,陈荣华.人胚胎肾间质成纤维细胞培养及其鉴定[J].南京医科大学学报,2003,23(1):11-13,F003. |
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| 作者姓名： | 潘晓勤 黄文彦 陈荣华 |
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| 作者单位： | 潘晓勤（南京医科大学小儿肾脏病研究中心,江苏,南京,210029）
黄文彦（南京医科大学小儿肾脏病研究中心,江苏,南京,210029）
陈荣华（南京医科大学小儿肾脏病研究中心,江苏,南京,210029） |
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| 基金项目： | 江苏省教委科研基金资助项目(99KJD320011) |
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| 摘 要： | 目的：探讨人胚胎肾脏成纤维细胞培养方法。方法：利用人胚胎肾脏肾髓质进行培养，然后通过细胞形态学，超微结构，免疫细胞化学等方法进行分析鉴定。结果：培养的细胞为长梭形，单核，超微结构为典型的成纤维细胞，并表达成纤维细胞表面抗原，波形蛋白，结蛋白，不表达角蛋白。结论：成功地培养出人胚胎肾间质成纤维细胞。
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| 关 键 词： | 成纤维细胞 细胞培养 肾脏 胚胎 细胞形态学 超微结构 免疫细胞化学 肾间质纤维化 肾功能衰竭 |
| 文章编号： | 1007-4368(2003)01-0011-03 |
Culture and Identification of Human Embryo Renal Interstitial Fibroblasts |
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| PAN Xiao-qin,HUANG Wen-yan,CHEN Rong-hua.Culture and Identification of Human Embryo Renal Interstitial Fibroblasts[J].Acta Universitatis Medicinalis Nanjing,2003,23(1):11-13,F003. |
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| Authors: | PAN Xiao-qin HUANG Wen-yan CHEN Rong-hua |
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| Abstract: | Objective: To explore the method to culture human fetal renal interstitial fibrqblasts in vitro. Methods: The renal interstitial fibroblasts from human fetal kidney grew up in culture median. They were identified by morphology, ultrastructure examination and immunocytochemistry. Results: (1) The culture cells were fusiform mononuclear mono-nuclei cells with typical ultrastructure of fibroblast under transmission electron microscope. (2) The cells expressed vimentin, desmin and fibroblast surface antigen positively, but no expression of keratin. Conclusion: The method was successfully utilized to culture highly pure human fetal renal interstitial fibroblasts. |
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| Keywords: | fibroblast cell culture kidney embryo |
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