| 响应面优化酶法提取平菇多糖工艺研究 |
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| 引用本文： | 钱磊,张春椿,蒋剑平,熊耀康.响应面优化酶法提取平菇多糖工艺研究[J].中华中医药学刊,2011(3):650-653. |
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| 作者姓名： | 钱磊 张春椿 蒋剑平 熊耀康 |
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| 作者单位： | 浙江中医药大学;浙江中医药大学附属第一医院; |
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| 摘 要： | 目的:建立平菇多糖的含量测定方法,确定纤维素酶法提取平菇多糖的最佳工艺条件。方法:采用苯酚-硫酸法测定平菇多糖的含量。以平菇多糖得率为指标,考察酶解时间、酶解温度、pH值等3个因素对平菇多糖纤维素酶提取工艺的影响,采用响应面分析法优化工艺条件。结果:平菇多糖的最佳提取工艺条件为酶解时间135min、酶解温度49℃、pH5.30,在此条件下得率可达6.82%。结论该法成本低、产品得率高,比传统水提法提高3.98%。
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| 关 键 词： | 纤维素酶法 平菇 多糖 响应面分析法 |
Optimization of Enzymatic Extraction Technology of Polysaccharides from Pleurotus ostreatus by Response Surface Methodology |
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| QIAN Lei,ZHANG Chun-chun,JIANG Jian-ping,XIONG Yao-kang.Optimization of Enzymatic Extraction Technology of Polysaccharides from Pleurotus ostreatus by Response Surface Methodology[J].Chinese Archives of Traditional Chinese Medicine,2011(3):650-653. |
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| Authors: | QIAN Lei ZHANG Chun-chun JIANG Jian-ping XIONG Yao-kang |
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| Institution: | QIAN Lei1,ZHANG Chun-chun1,JIANG Jian-ping1,2,XIONG Yao-kang1(1.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China,2.The Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310007,China) |
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| Abstract: | Objective:To establish determination methods and optimal extraction conditions of polysaccharides from Pleurotus ostreatus treated with cellulase.Methods:Total carbohydrate content was determined by the phenol-sulfuric acid method and the yield of polysaccharides was the indice to determine extraction process by a combination of response surface methodology(RSM).the enzymolysis time,enzymolysis temperature and pH value were selected as factors and a three-factors-three-levels experiment was developed by Box... |
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| Keywords: | cellulase Pleurotus ostreatus polysaccharides response surface methodology |
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