| 六味地黄丸防治大鼠糖尿病肾病的实验研究 |
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| 引用本文： | 李志杰,张悦,刘煜敏,陆海英,李亚丽,张燕.六味地黄丸防治大鼠糖尿病肾病的实验研究[J].中华中医药学刊,2011(8):1728-1731,1929. |
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| 作者姓名： | 李志杰 张悦 刘煜敏 陆海英 李亚丽 张燕 |
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| 作者单位： | 上海中医药大学; |
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| 基金项目： | 国家自然科学基金资助项目(30371834); 上海市教育委员会重点学科(第5期)项目(J50301); 上海高校创新团队建设项目; 上海市高校优秀青年教师专项基金资助项目 |
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| 摘 要： | 目的:观察足细胞裂孔隔膜蛋白nephrin和podocin在糖尿病肾病发病机制中的作用,探讨六味地黄丸(LWDHW)保护糖尿病肾病大鼠肾脏的机制。方法:SD大鼠腹腔注射链脲佐菌素复制糖尿病模型,1周后,按血糖浓度将成模的糖尿病大鼠随机分为:模型组(STZ,8只)、治疗组(STZ+LWDHW,8只),另设正常组(Control,8只)。治疗组给予六味地黄丸灌胃,模型组、正常组给予等量生理盐水,持续观察8周。治疗第8周末,称大鼠体重,计算肾系数,观察肾脏病理改变;检测24h尿蛋白总量、血尿素氮、血肌酐;免疫印迹检测nephrin和podocin在肾组织中的表达。结果:与正常组比较,模型组大鼠血糖,体重明显下降(P<0.01),肾系数,24h尿蛋白总量,血尿素氮,血肌酐明显升高(P<0.01);模型组大鼠肾小球肥大、毛细血管基底膜增厚,系膜基质增生,肾小管上皮细胞空泡样变,见蛋白管型;nephrin和podocin蛋白表达减少(P<0.01)。治疗组血糖、体重无明显改变;肾系数,24h尿蛋白总量,血尿素氮,血肌酐明显降低(P<0.05);病理改变明显减轻;大鼠肾组织nephrin和podocin表达明显增加(P<0.05)。结论:六味地黄丸可能通过增加足细胞nephrin和podocin表达发挥保护糖尿病肾病大鼠肾脏的作用。
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| 关 键 词： | 糖尿病肾病 六味地黄丸 nephrin podocin |
Empirical Study on Prevention and Treatment of Liu Wei Di Huang Wan in Diabetic Nephropathy Rats |
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| LI Zhi-jie,ZHANG Yue,LIU Yu-min,LU Hai-ying,LI Ya-li,ZHANG Yan.Empirical Study on Prevention and Treatment of Liu Wei Di Huang Wan in Diabetic Nephropathy Rats[J].Chinese Archives of Traditional Chinese Medicine,2011(8):1728-1731,1929. |
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| Authors: | LI Zhi-jie ZHANG Yue LIU Yu-min LU Hai-ying LI Ya-li ZHANG Yan |
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| Institution: | LI Zhi-jie,ZHANG Yue,LIU Yu-min,LU Hai-ying,LI Ya-li,ZHANG Yan(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) |
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| Abstract: | Objcetivt: To investigate the role of the nephrin and podocin in podocytes slit diaphragm in diabetic nephropathy(DN) pathogenesis and study the mechanism of prevention and treatment this disease the effects of Liu Wei Di Huang Wan.Methods:The rat model of diabetes was induced by intraperitoneal injection of Streptozotocin(STZ),and diabetic rats were randomly divided into 2 groups by blood glucose concentration: STZ group(n=8) and LWDHW group(n=8).In addition,8 non-treated rats served as control group.All t... |
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| Keywords: | diabetic nephropathy Liu Wei Di Huang Wan nephrin podocin |
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