| 三七总皂苷对缺氧再给氧血管内皮细胞钙离子浓度及肌浆网Ca2+-ATP酶活性的影响 |
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| 引用本文： | 江小萍,李海全,潘少霞,方永奇,刘桂娥,欧阳永红,沈祖泓.三七总皂苷对缺氧再给氧血管内皮细胞钙离子浓度及肌浆网Ca2+-ATP酶活性的影响[J].中华中医药学刊,2011(4):758-760. |
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| 作者姓名： | 江小萍 李海全 潘少霞 方永奇 刘桂娥 欧阳永红 沈祖泓 |
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| 作者单位： | 清远市中医院内一科;清远市中医院检验科;广州中医药大学第一临床学院中心实验室; |
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| 基金项目： | 广东省中医药局建设中医药强省科研基金资助项目(2007292) |
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| 摘 要： | 目的:探讨人脐静脉内皮细胞株(ECV304)缺氧再给氧(H/R)损伤的发生机制及三七总皂苷(tPNS)对该病理生理过程的影响。方法:将体外培养的ECV304分为七组,正常组;模型组;tPNS高、中、低浓度组;维拉帕米组;吡咯烷二硫氨基甲酸酯(PDTC)组,对各组进行相应处理后,分别检测各组细胞内钙离子浓度Ca2+]i、肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力的变化。结果:模型组、tPNS高、中、低浓度组、维拉帕米组及PDTC组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显低于正常组,Ca2+]i明显高于正常组,其中tPNS高、中、低浓度组、维拉帕米组及PDTC组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显高于H/R组,Ca2+]i明显低于H/R组,且呈浓度依赖性改变,tPNS高浓度组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显高于维拉帕米组及PDTC组,Ca2+]i明显低于维拉帕米组及PDTC组。结论:H/R可激活ECV304,使细胞肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显降低,Ca2+]i明显升高;tPNS能有效改善肌浆网Ca2+-ATP酶活性,减轻细胞内钙超载,减轻H/R损伤,且高浓度tPNS效果优于维拉帕米、PDTC。
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| 关 键 词： | 人脐静脉内皮细胞株ECV304 缺氧再给氧损伤 钙离子 Ca2+-ATP酶 三七总皂苷 |
The Effect of Total Panax Notoginseng Saponins on Calcium Ion and Sarcoplasmic Reticulum Ca~(2+)-ATP Ase of ECV304 in Hypoxia/reoxygenation Injury |
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| JIANG Xiao-ping,LI Hai-quan,PAN Shao-xia,FANG Yong-qi,LIU Gui-e,OUYANG Yong-hong,SHEN Zu-hong.The Effect of Total Panax Notoginseng Saponins on Calcium Ion and Sarcoplasmic Reticulum Ca~(2+)-ATP Ase of ECV304 in Hypoxia/reoxygenation Injury[J].Chinese Archives of Traditional Chinese Medicine,2011(4):758-760. |
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| Authors: | JIANG Xiao-ping LI Hai-quan PAN Shao-xia FANG Yong-qi LIU Gui-e OUYANG Yong-hong SHEN Zu-hong |
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| Affiliation: | JIANG Xiao-ping1,LI Hai-quan1,PAN Shao-xia2,FANG Yong-qi3,LIU Gui-e1,OUYANG Yong-hong1,SHEN Zu-hong1(1.The Chinese Traditional Hospital of Qingyuan city,physical department one,Qingyuan 511500,Guangdong,China,2.The Chinese Traditional Hospital of Qingyuan city,laboratory,3.The Central Laboratory of the First Clinical Medical College Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China) |
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| Abstract: | Objective:To study the mechanisms of ECV304 with hypoxia/reo-xygenation(H/R) injury and the effect of total panax notoginseng saponins(tPNS) on the pathophysiologic process.Methods:Divided the culture ECV304 to 7 groups,control group;H/R group;high,middle and low concentrations of tPNS group;Verapamil group;PDTC group.Every group are taked corresponding disposition.The ECV304 i,sarcoplasmic reticulum Ca2+-ATP ase were detected,The content of NO and PGI2,Cell viability were measured.Result:The cell of sarcop... |
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| Keywords: | ECV304 hypoxia/reoxygenation Calcium ion Ca2 -ATP ase total panax notoginseng saponins |
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