| 高效液相色谱法测定嗣育保胎丸中芍药苷的含量 |
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| 引用本文： | 水彩红,曹红,陈玉敏,沈国梁.高效液相色谱法测定嗣育保胎丸中芍药苷的含量[J].解放军药学学报,2010,26(5):434-435. |
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| 作者姓名： | 水彩红 曹红 陈玉敏 沈国梁 |
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| 作者单位： | 1. 总后勤部卫生部药品仪器检验所,北京,100071
2. 北京昌平南口66325部队药学部,北京,102202 |
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| 摘 要： | 目的建立嗣育保胎丸中芍药苷的含量测定方法。方法采用高效液相色谱法,以岛津VP-ODS（4.6mm×250mm,5μm）为色谱柱;流动相：乙腈-0.1%磷酸溶液（14：86）;检测波长：230nm;柱温：40℃。结果芍药苷在0.1130～1.6950μg范围内,浓度与峰面积线性关系良好（r=0.9996）;样品的平均加样回收率为100.00%,RSD为1.59%,n=6。结论本文方法简便、快速,可作为嗣育保胎丸中芍药苷的含量测定方法。
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| 关 键 词： | 高效液相色谱法 芍药苷 嗣育保胎丸 |
Determination of Paeoniflorin in Siyubaotaiwan by High Performance Liquid Chromatography |
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| SHUI Cai-hong,CAO Hong,CHEN Yu-min,SHEN Guo-liang.Determination of Paeoniflorin in Siyubaotaiwan by High Performance Liquid Chromatography[J].Pharmaceutical Journal of Chinese People's Liberation Army,2010,26(5):434-435. |
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| Authors: | SHUI Cai-hong CAO Hong CHEN Yu-min SHEN Guo-liang |
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| Institution: | (①Institute for Drug and Instrument Control of Health Dept GLD of PLA,Beijing100071,China②Health Department of Unit 66325 of PLA,Beijing102202,China ) |
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| Abstract: | Objective To establish a method for determination of paeoniflorin in Siyubaotaiwan.Methods High performance liquid chromatography was used,wiht SHIMADZU VP-ODS（4.6 mm×250 mm,5 μm） as the stationary phase,and acetonitrile-0.1% phosphoric acid（14：86） as the mobile phase.The photodiode array detector was used at 230 nm.Column temperatuer was 40℃.Results The calibration curve was linear over the range of 0.1130-1.6950 μg （r=0.9996）.The average recovery of the method was 100.00%,RSD 1.59%（n=6）.Conclusion This method is reliable,accurate and can be applied to separate and determine paeoniflorin in Siyubaotaiwan. |
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| Keywords: | high performance liquid chromatography Paeoniflorin Siyubaotaiwan |
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