| 大鼠坐骨神经损伤后脑源性神经营养因子对脊髓前角内生长相关蛋白GAP-43表达的影响 |
| |
| 引用本文： | 王知非,廖达光,李昌琪. 大鼠坐骨神经损伤后脑源性神经营养因子对脊髓前角内生长相关蛋白GAP-43表达的影响[J]. 南方医科大学学报, 2010, 30(3): 569 |
| |
| 作者姓名： | 王知非 廖达光 李昌琪 |
| |
| 作者单位： | 中南大学湘雅三医院神经外科,湖南,长沙,410013;中南大学湘雅医学院人体解剖与神经生物学系,湖南长沙,410013 |
| |
| 摘 要： | 目的探讨大鼠坐骨神经损伤后内源性脑源性神经营养因子(BDNF)对脊髓前角内生长相关蛋白GAP-43表达的调节。方法大鼠坐骨神经压榨损伤后,腹腔注射BDNF抗体中和内源性BDNF,对照组注射生理盐水,动物存活7d或14d,用Western blot与RT-PCR观察GAP-43在脊髓腰骶膨大部前角的表达。结果与对照组相比,注射BDNF抗体后坐骨神经损伤侧脊髓前角内GAP-43蛋白与其mRNA的表达明显下降,上述改变在统计学上均有显著意义(P<0.01)。结论大鼠坐骨神经损伤后内源性BDNF可能参与脊髓前角内GAP-43表达的调节。
|
| 关 键 词： | 脑源性神经营养因子 生长相关蛋白-43 脊髓 坐骨神经 大鼠 |
Effect of endogenous brain derived neurotrophic factor on GAP-43 expression in the anterior horn of the spinal cord in rats with sciatic nerve injury |
| |
| WANG Zhi-fei,LIAO Da-guang,Li Chang-qi. Effect of endogenous brain derived neurotrophic factor on GAP-43 expression in the anterior horn of the spinal cord in rats with sciatic nerve injury[J]. Journal of Southern Medical University, 2010, 30(3): 569 |
| |
| Authors: | WANG Zhi-fei LIAO Da-guang Li Chang-qi |
| |
| Affiliation: | WANG Zhi-fei1,LIAO Da-guang1,Li Chang-qi2 Department of Neurosurgery,Third Xiangya Hospital1,Department of Anatomy , Neurobiology,Xiang-ya College of Medicine2,Central South University,Changsha 410013,China |
| |
| Abstract: | Objective To investigate the effect of endogenous brain derived neurotrophic factor (BDNF) on GAP-43 expression in the anterior horn of the spinal cord in rats following sciatic nerve injury. Methods BDNF antibody was injected intraperitoneally in rats with crushing injury of the sciatic nerve, and the control rats received normal saline only after sciatic nerve injury. At 7 and 14 days after the injection, the expression of GAP-43 in the anterior horn of the corresponding segments of the spinal cord was de... |
| |
| Keywords: | brain-derived neurotrophic factor GAP-43 spinal cord sciatic nerve rats |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 |
|
点击此处可从《南方医科大学学报》下载全文 |
|
