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Recombinant E. coli LLO/OVA induces murine BMDCs maturation via TLR4 and NOD1 receptor and promotes specific cytotoxic T cell immunity |
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| Authors: | Xu Man Dai Ming-Shen |
| Institution: | MAN XU,2 AND MING-SHEN DAI# Department of Pathology,Molecular Medicine and Cancer Research Centre,Chongqing Medical University,Chongqing 400016,China,#Centre for Molecular Oncology,Institute of Cancer,Queen Mary's School of Medicine and Dentistry,London EC1M 6BQ,UK |
| Abstract: | Objective To explore the immune stimulation effect of recombinant E.coli LLO/OVA on mice bone marrow-derived dendritic cells (BMDCs) and T lymphocytes in vitro.Methods After BMDCs stimulated by E.coli LLO/OVA,their Toll-like receptor (TLR) and nucleotide-binding oligomerization domain (NOD) receptor signalling pathway were examined by superarray hybridization;and the priming effect of the vaccine activated BMDCs on CD4+T and CD8+T was determined by 3H]thymidine uptake and ELISA,the tumor cytotoxic effect o... |
| Keywords: | Bone marrow-derived dendritic cells BMDCs Recombinant Escherichia coli Toll-like receptor (TLR) Nucleotide-binding oligomerization domain (NOD) CD8 T cells |
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