Inhibition of influenza C viruses by human MxA protein |
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Authors: | Marschall M Zach A Hechtfischer A Foerst G Meier-Ewert H Haller O |
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Institution: | Institut für Klinische und Molekulare Virologie, Universit?t Erlangen-Nürnberg, Germany. mdmarsch@viro.med.uni-erlangen.de |
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Abstract: | Human MxA protein was analyzed for its ability to inhibit the replication of different influenza C viruses. Three laboratory derivatives of viral strain C/Ann Arbor/1/50 were investigated, namely the parental wild-type virus C/AA-wt, the persistent variant C/AA-pi and the highly cytopathogenic variant C/AA-cyt. In addition, strain C/Paris/214/91 isolated from an influenza patient was used. Multiplication of all four viruses was suppressed in MxA-expressing Vero cells, as indicated by a decrease in viral RNA synthesis, viral protein synthesis, virion production and induction of a cytopathic effect. Inhibition correlated with the level of MxA expression. Furthermore, inhibition was independent of cell clone-specific differences in expression of virus receptors, as demonstrated by receptor reconstitution experiments. Thus, human MxA protein has antiviral activity against influenza C viruses. |
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