体外视网膜母细胞瘤细胞对不同化疗药物的敏感性分析

[目的]研究视网膜母细胞瘤(retinoblastma,RB)细胞对化疗药物的体外敏感性,以制定合理的RB临床个体化化疗方案.[方法]应用3-(4,5)-双甲基-2噻唑-(2,5)-二甲基溴化四氮唑蓝(MTT)体外药敏法,测定35例RB患儿瘤细胞对常用的阿霉素、长春新碱、鬼臼乙叉苷、卡铂、甲氨喋呤、放线菌素D和噻替哌共7种化疗药物的敏感性.[结果]RB的药敏可评价率是86.7%.同一种药物对不同的肿瘤细胞有不同的抑制率,7种药物的最高抑制率是卡铂(91.8%)、噻替哌(90.7%)、放线菌素D(89.7%)、鬼臼乙叉苷(88.4%)、阿霉素(86.61%)、甲氨喋呤(58.66%)、长春新碱(54.95%);最低的直接抑制率均是0.[结论]不同RB个体的瘤细胞对不同的化疗药物有不同的敏感性,体外药敏试验筛选敏感化疗药物对临床RB个体化治疗有应用价值.

Analysis of Drug Sensitivity of Variant Antineoplastic Drugs on Retinoblastoma Cells In Vitro

[Objective] To investigate the chemosensitivity of retinoblastoma (RB) cells in vitro in order to set reasonable and individual chemotherapy project. [Methods] Using 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay to detect the chemosensitivity of 7 ordinary antineoplastic drugs, doxorubicin, vincrinstine, etopside, carboplatin, methotrexate, actinomycin and thiotepa acting on retinoblastoma cells. [Results] The MTT assay was successful in 86.7% of the retinoblastoma samples. The same drug has different inhibiting rate to different retinoblastoma cells. The maximum of inhibiting rate was 91.8% of carboplatin, 90.7% of thiotepa, 89.7% of actinomycin, 88.4% of etopside, 86.61% of doxorubcin, 58.66% of methotrexate, 54.95% of vincrinstine and the minimum of inhibiting rate was 0 of all the 7 antineoplastic drugs. [Conclusion] The different RB samples have different drug sensitivity to variant antineoplastic drugs. The drug sensitive test has certain applying value in individual treatment to select sensitive drugs for patients with RB.