乙酰唑胺对缺氧耐受小鼠血清T-chE和T-AOC的影响

目的：研究乙酰唑胺对小鼠缺氧耐受的影响和机制。方法：小鼠随机分四组：生理盐水对照组、心得安对照组、乙酰唑胺高剂量组和乙酰唑胺低剂量组。高剂量组予以乙酰唑胺1g/kg、低剂量组予以乙酰唑胺0．2g／kg灌胃，阳性对照组给予心得安0．16g/kg灌胃，正常对照组用20g/kg生理盐水灌胃，各组小鼠均每天灌胃一次，连续5天并于末次给药1小时后测定小鼠在室温空调25℃条件下的常压缺氧耐受能力；同时在小鼠死亡时尽快断头取血，以3000r／min，离心5分钟，按试剂盒说明书测定血清胆碱酯酶（T-ChE）活性和总抗氧化能力（T-AOC）。结果：乙酰唑胺低剂量组和高剂量组小鼠耗氧率均较生理盐水组显著降低（均P〈0．01），与心得安组比较差异有显著性（分别P〈0．01和P〈0．05）；乙酰唑胺高剂量组T-ChE活性和T—AOC均明显比生理盐水组高（均P〈0．01），比心得安组显著增高（分别P〈0．01和P〈0．05）。结论：乙酰唑胺高、低剂量均能提高小鼠抗常压的缺氧耐受能力，与提高T-AOC和T-ChE活性有关。

Effects of acetazolamide on the ability of anti-anoxia and the vitality of T-ChE and T-AOC in serum in mice

Objective: To study the effects and mechanism of acetazolamide on the ability of anti-anoxia in mice.Methods:Mice were divided into four groups at random.Acetazolamide high dose group was intragastric administration with acetazolamide 0.1 g/kg/d but the low dose 0.02 g/kg/d,Propranolol group was intragastric administration with Propranolol 0.16 g/kg/d,and saline group was intragastric administration with saline 20 g/kg/d,all the mice were treatmed for 5 days. At last time,the mice were put in a jar to test the ability of anti-anoxia after intragastric administration for 1 hour,and the serum activity of T-ChE and T-AOC was measured respectively.Results:Both of acetazolamide high dose and low dose groups were improved the ability of ant-anoxia very significantly(both P<0.01),and the T-ChE and T-AOC activities of the serum were improved significantly (all P<0.01). Conclusion:The acetazolamide can improve the ability of anti-anoxia by improving the T-AOC and T-ChE activities.