595nm激光对兔耳瘢痕成纤维细胞增殖与凋亡的影响

目的：探讨595nmVbeam激光照射对增生性瘢痕动物模型伤口愈合过程中成纤维细胞增殖与凋亡的影响。方法：成年大耳白兔20只,建立兔耳腹侧面增生性瘢痕模型,对比研究在瘢痕形成过程中595nmVbeam激光照射对兔耳瘢痕成纤维细胞的影响,采用免疫组化方法检测增殖细胞核抗原（PCNA）蛋白表达和细胞凋亡的原位检测。结果：兔耳增生性瘢痕经595nmVbeam激光照射后,按不同时间段取材进行免疫组化染色并与对照组比较,高倍镜下观察结果,显示PCNA蛋白表达明显减弱,细胞凋亡增加。结论：595nmVbeam激光照射可抑制兔耳增生性瘢痕成纤维细胞的增殖过程,诱导细胞凋亡。应用595nmVbeam激光预防和治疗瘢痕是可行的。

The effect of 595nm Vbeam laser on proliferative activity and apoptosis of fibroblasts in the hypertrophic scarring models in rabbit ears

Objective To investigate the effect of 595nm Vbeam laser on proliferative activity and apoptosis of fibroblasts in the hypertrophic scarring models in rabbit ears. Methods 20 white rabbits were used to establish the models of hypertrophic scar in ears. To study the changes of fibroblasts with 595nm Vbeam laser irradiation and compare with the controls during the process of forming hypertrophic scars. The expression of proliferation cell nuclear antigen (PCNA) protein was measured by immunohistochemistry technique, and the apoptosis was measured by TUNEL technique. Results After 595nm Vbeam laser irradiation, the material was taken on different time. The expression of PCNA was obviously weaker than the controls, and the apoptosis was increased in the laser irradiation group. Conclusion 595nm Vbeam laser irradiation can inhibit the proliferation of fibroblasts and accelerate apoptosis in hypertrophic scars of rabbit ears and has significant effect on hypertrophic scars.