氧自由基在肝脏保存再灌注损伤中的作用及丹参的保护作用

目的 探讨氧自由基在肝脏保存再灌注损伤（ｈｅｐａｔｉｃ ｐｒｅｓｅｒｖａｔｉｏｎ ｒｅｐｅｒｆｕｓｉｏｎ ｉｎｊｕｒｙ，ＨＰＲＩ）中的作用及丹参的保护作用。方法 建立大鼠肝脏保存再灌注模型，分别检测肝脏保存０、８、１６、２４、３２小时组在不同灌注时间点组织超氧化物酶（ＳＯＤ）、丙二醛（ＭＤＡ）和灌注液俗 草转氨酸（ＡＳＴ）的变化，并观察肝细胞形态学的改变。结果 与正常组（保存０小时组）比较，保存１

The effects of salviamiltiorrhiza on the role of oxygen free radicals in hepatic preservation reperfusion injury

Objective To explore the role of oxygen free radicals (OFR) in hepatic preservation reperfusion injury (HPRI) of rats and the protective effect of salviamiltiorrhiza (SM). Methods The model of the preservation reperfusion of isolated rat liver was established. The changes of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver and aspartate aminotransferase (AST) in perfusion fluid were studied at different preservation reperfusion time points (0,8,16,24,32 h), and morphological changes of hepatocytes were observed. Results The activity of SOD was decreased ( P <0.05) and MDA and AST levels increased significantly ( P <0.05) at the liver preservation reperfusion points of 16, 24 and 32 h as compared with those of normal group. Abnormal changes of hepatocytes were found in morphology. After the administration of SM, SOD was obviously increased, and MDA and AST significantly decreased as compared with those in control group ( P <0.05). Conclusion OFR may be one of the primary causes for HPRI. SM can attenuate HPRI by eliminating OFR.;