正常小鼠冠状动脉的超声成像技术分析

目的探讨利用高频小动物心脏超声对C57BL/6小鼠冠状动脉进行评价的可行性,为小鼠冠状动脉相关疾病动物模型的制备及其功能评价提供依据。方法采用Vevo 770型高分辨小动物超声仪,频率30 mHz的宽频探头,对20只健康C57BL/6小鼠于4、8和12周龄时冠状动脉的情况进行观察。测定和分析不同周龄小鼠冠状动脉内径值的变化。结果全部20只小鼠超声均成功检测到冠状动脉。超声心动图显示小鼠4周龄时左冠状动脉主干内径检测值为0.36±0.02 mm,右冠状动脉主干内径值为0.29±0.03 mm;8周龄时左冠状动脉主干内径值为0.38±0.06 mm,右冠状动脉主干内径值为0.37±0.02(mm);12周龄时左冠状动脉主干内径值为0.38±0.02 mm,右冠状动脉主干内径值为0.39±0.03 mm。结论利用高频小动物心脏超声可获取正常小鼠清晰的冠状动脉图像,并能准确反映小鼠冠状动脉内径值动态变化。为小鼠冠状动脉疾病模型的制备及其功能评价提供依据。

Analysis of coronary artery imaging in healthy mice with Echocardiography

Objective The aims of this study were to assess the feasibility of coronary artery in C57BL/6 mice With a high frequency ultrasound system and to provide evidence for preparation the murine model of KD. Methods Totally 20 mice were chosen and Visualsonics Vevo 770 UBM was used in this study. On weeks 4, 8 and 12, the developmental changes and dimensions of coronary artery of the mice were measured by echocardiography. Results By echocardiography, the present study successfully detected coronary artery diameter in the murine model of KD. On week 4, the diameter of left coronary artery in the mice was 0.32?.14 mm, and the diameter of right coronary artery was 0.37?.02 mm, On week 8, the diameter of left coronary artery in the mice was 0.38?.06 mm, and the diameter of right coronary artery was 0.37?.02 mm; on week 12, the diameter of left coronary artery in the mice was 0.38?.02 mm, and the diameter of right coronary artery was 0.39?.03 mm. Conclusions The present study successfully detected coronary arteries by echocardiography, and the diameter of coronary artery in the mice could be measured which way be benefit for the study in the murine model of coronary arteries disease.