| 免疫印迹检测大疱性类天疱疮抗体 |
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| 引用本文： | 张学军 翁孟武. 免疫印迹检测大疱性类天疱疮抗体[J]. 中国免疫学杂志, 1995, 11(1): 48-50 |
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| 作者姓名： | 张学军 翁孟武 |
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| 作者单位： | 上海医科大学皮肤病学研究所 |
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| 摘 要： | 免疫印迹检测３６例大疱性类天疱疮（ＢＰ）患者血清中类天疱疮抗体（ＢＰＡｂ）。结果表明，３２例（８９％）ＢＰ血清与正常人皮肤热分离表皮的提取物中２３０ｋＤ、１９０ｋＤ、１６５ｋＤ、１４５ｋＤ和１１５ｋＤ多肽反应，其中１６例（４４％）血清与２３０ｋＤ和１６５ｋＤ多肽同时反应，７例（１９％）血清单独与２３０ｋＤ多肽反应，９例（２５％）血清单独与１６５ｋＤ多肽反应。结果提示，免疫印迹检测ＢＰＡｂ可用于ＢＰ诊断。
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| 关 键 词： | 免疫印迹 类天疱疮 抗体 大疱性 |
DETECTION OF THE BULLOUS PEMPHIGOID ANTIBODIES BYIMMUNOBLOTTING |
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| Zhang Xuejun,Weng Mengwu,Shi Shouyi Institute of dermatology,shanghai medical univer-sity,Shanghai. DETECTION OF THE BULLOUS PEMPHIGOID ANTIBODIES BYIMMUNOBLOTTING[J]. Chinese Journal of Immunology, 1995, 11(1): 48-50 |
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| Authors: | Zhang Xuejun Weng Mengwu Shi Shouyi Institute of dermatology shanghai medical univer-sity Shanghai |
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| Affiliation: | Zhang Xuejun,Weng Mengwu,Shi Shouyi Institute of dermatology,shanghai medical univer-sity,Shanghai 200040 |
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| Abstract: | We detected the bullous pemphigoid antibodies(BP-Ab)of sera from 3 6 patients with bul-lous pemphigoid (BP)and 20 control subjects(normal volunteers,pemphigus vulgaris , epider-molysis bullosa acquisita )by immunoblotting,32(89%)sera reacted with 230 kD,190 kD,165kD,145 kD and 115 kD antigens from human heat-separated epidermis. The different types ofreactivity couid be defined:(1)the presence of 230 kD aiitigen exclusively in 7(19%)sera;(2)the presence of 165 kD antigen excltisively in 9(25%) sera;(3)labelling of the 230kD and165 kD antigen by same serum in 16(44%)sera,The results suggest that detection of Bp-Ab byimmunoblotting is used for the diagnosis of BP. |
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| Keywords: | Immunoblotting Bullous pemphigoid dntibodies |
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