Evaluation of a novel lentiviral vaccine expressing KMP11‐HASPB fusion protein against Leishmania infantum in BALB/c mice |
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| Authors: | N. Mortazavidehkordi A. Farjadfar H. Khanahmad Z. Ghayour Najafabadi N. Hashemi A. Fallah A. Najafi V. Kia S. H. Hejazi |
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| Affiliation: | 1. Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran;2. Biotechnology Research Center, Mede Bioeconomy Company, Tehran, Iran;3. Department of Genetics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;4. Systems and Synthetic Biology Group, Mede Bioeconomy Company, Tehran, Iran;5. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran;6. Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran;7. Skin Diseases and Leishmaniais Research Center, Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran |
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| Abstract: | Hydrophilic acylated surface protein B (HASPB) is an immunogenic Leishmania protein against which antibodies are produced in the sera of cutaneous and visceral Leishmaniasis (VL) patients. Kinetoplastid membrane protein 11 (KMP11) is another protein antigen of Leishmania which is reported as a promising candidate for vaccination of VL. It is a highly conserved surface protein present in all members of kinetoplastid family and is expressed in both promastigotes and amastigotes. In this study, the coding sequence of KMP11 and HASPB was cloned into a pCDH‐cGFP lentiviral vector as a fusion protein. The gene expression was confirmed using RT‐PCR and Western blot methods. After injection of the recombinant KMP11‐HASPB‐expressing lentiviruses to BALB/c mice, using ELISA technique, a significant increase in IFN‐γ and IL‐4 as well as IgG1 and IgG2a was observed compared to the control group. Furthermore, the number of parasites in the liver and spleen of vaccinated mice decreased significantly compared with the control group. |
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| Keywords: |
HASPB
KMP11 recombinant lentiviral vaccination |
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