急性放射病患者恢复期外周血淋巴细胞表型及功能分析

了解急性放射病患者恢复期间外周血淋巴细胞功能。方法用流式细胞仪分析淋巴细胞表型，３Ｈ－ＴｄＲ掺入法分别分析Ｔ细胞和ＮＫ细胞功能。结果照后４．５年，患者外周血ＣＤ＋４Ｔ细胞仍有不同程度低于正常对照；受照剂量大于２Ｇｙ者ＣＤ＋８Ｔ细胞数明显高于正常，而受照时年龄为５４岁者ＣＤ＋８Ｔ细胞明显低于对照；多数患者外周血ＮＫ细胞数和功能都高于正常对照。结论受照者外周血Ｔ细胞功能恢复与受照剂量大小及年龄因素有关，恢复期ＮＫ细胞数量和活性增高，对于弥补Ｔ细胞功能不足具有积极意义

Analysis of phenotype and function of peripheral blood lymphocytes for patients with acute radiation sickness during recovery stage

Objective To research the peripheral blood lymphocyte functions in patients with acute radiation sickness during recovery stage. Methods Phenotype of lymphocytes was analysed with flow cytometry;the functions of T cells and NK cells were analysed by biological method through determination of ³H TdR incorporation. Results At four and a half years after radiation exposure the CD₄⁺ T cell counts in patients' peripheral blood was still lower than those in normal controls;the patients whose exposure dose was over 4 Gy showed higher PB CD₈⁺ T cell counts but a patient exposed at the age of 54 years showed obviously lower counts;during restoration, all patients had enhanced CD ⁺ 16 cell count and NK cell function. Conclusion The restoration of T cell functions for the patients seemed associated with exposure dose and patients' age at the time of exposure;the enhancement in the number and activity of the NK cells for the patients during restoration may play a significant role in cellular immune recovery.