三氯乙烯诱发豚鼠过敏性皮炎的免疫学效应

目的研究三氯乙烯(TCE)诱发豚鼠过敏性皮炎的免疫学效应。方法采用豚鼠最大值法建立TCE致敏动物模型,应用ELISA双抗体夹心法定量检测血清中IgG含量;用MTT法测定刀豆蛋白A刺激下T淋巴细胞的增殖转化功能,用乳酸脱氢酶释放法测定NK细胞功能。结果TCE的致敏率为667%,属强致敏物。TCE致敏豚鼠血清总IgG含量明显高于阴性对照组(P<005)。TCE致敏豚鼠脾T淋巴细胞增殖转化功能及NK细胞对瘤细胞的杀伤活性与对照组相比无显著性差异(P>005)。结论体液免疫在TCE诱发过敏性皮炎过程中发挥一定的作用。在本染毒方式和染毒剂量下,未见TCE对豚鼠T淋巴细胞和NK细胞活性的影响。

The immunological effects in guinea pig sensitized by trichloroethylene

Objective To investigate the changes of certain immunological indexes in development of Guinea Pig allergic dermatitis induced by trichloroethylene (TCE). Methods The Guinea Pig model of TCE-induced allergic dermatitis was established by Guinea Pig Maximisation Test. IgG levels in serum were measured by sandwich enzyme immunoassay. The splenic T lymphocytes ConA-stimulated proliferation function and natural killer (NK) cell activity were detected by MTT assay and micro-LDH release assay, respectively. Results The sensitization rate of TCE was 66.7%. The IgG level in serum of TCE-sensitized animals was significantly higher than that in normal animals (P<0.05). No obvious differences in Splenic T lymphocytes proliferation index and NK cell activity between TCE-sensitized and normal animals were found(P>0.05). Conclusion Humoral immunity may play an important role in development of allergic dermatitis induced by TCE.