| 超临界流体色谱法测定三七及云南白药中人参二醇和人参三醇的含量 |
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| 引用本文： | 李云华,李修禄,虹岚,刘锦耀,张美义. 超临界流体色谱法测定三七及云南白药中人参二醇和人参三醇的含量[J]. 药学学报, 1991, 0(10) |
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| 作者姓名： | 李云华 李修禄 虹岚 刘锦耀 张美义 |
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| 作者单位： | 第二军医大学药学院，第二军医大学药学院，云南省分析测试研究所，云南省分析测试研究所，成都军区昆明总医院药局 上海 200433，上海 200433，昆明 650011，昆明 650011，昆明 650032 |
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| 摘 要： | 本文用超临界流体色谱法(SFC)测定了三七和云南白药中人参二醇及人参三醇的含量。使用15%硫酸乙醇—水(1:1)直接进行水解,然后碱水解,克服了文献报道的水解不完全、结果不稳定、不能定量回收的缺点。本法取样量少,灵敏度高,操作简单、快速、整个分析过程可在8h内完成。
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| 关 键 词： | 超临界流体色谱 人参二醇 人参三醇 三七 云南白药 |
DETERMINATION OF PANAXADIOL AND PANAXATRIOL IN RADIX NOTOGINSENG AND YUNNAN BAIYAO BY CAPILLARY SUPERCRITICAL FLUID CHROMATOGRAPHY |
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| YH Li,XL Li,L Hong,JY Liu and MY Zhang. DETERMINATION OF PANAXADIOL AND PANAXATRIOL IN RADIX NOTOGINSENG AND YUNNAN BAIYAO BY CAPILLARY SUPERCRITICAL FLUID CHROMATOGRAPHY[J]. Acta pharmaceutica Sinica, 1991, 0(10) |
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| Authors: | YH Li XL Li L Hong JY Liu MY Zhang |
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| Affiliation: | YH Li,XL Li,L Hong~*,JY Liu~* and MY Zhang~ |
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| Abstract: | Capillary supercritical fluid chromatography (SFC)was developed for the determination of panaxadiol and panaxatriol in Radix notoginseng and Yunnan Baiyao. 0.1 g Radix notoginseng powder or 0.5 g Yunnan Baiyao was mixed with 10 ml 15% H_2SO_4 ethanol- water (1:1 )solution, adding 1 mg cholesterol as internal standard. The mixture was refluxed for 4 h, then adding 15 ml 15% NaOH solution, refluxed for 0. 5 h.The mixture was extracted 3 times with 10 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by SFC. The proposed method is sensitive, accurate, precise, simple and rapid;all the process can be done in 8 h. |
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| Keywords: | Supercritical fluid chromatography Panaxadiol Panaxatriol Radix notoginseng Yunnan Baiyao |
| 本文献已被 CNKI 等数据库收录！ |
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