| 桂枝加减汤对MRL小鼠心肌细胞凋亡及Bax mRNA、Caspase3mRNA基因表达的影响 |
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| 引用本文： | 刘贵京,阎英杰,苗国英,陈兴起,于雪,张相玲,李增燕,王珍玉. 桂枝加减汤对MRL小鼠心肌细胞凋亡及Bax mRNA、Caspase3mRNA基因表达的影响[J]. 临床心血管病杂志, 2011, 27(7) |
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| 作者姓名： | 刘贵京 阎英杰 苗国英 陈兴起 于雪 张相玲 李增燕 王珍玉 |
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| 作者单位： | 1. 河北工程大学临床医学院,河北邯郸,056029
2. 新兴铸管职工医院 |
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| 基金项目： | 河北省教育厅基金资助课题 |
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| 摘 要： | 目的:探讨复方中药对MRL小鼠心肌细胞凋亡的作用及机理.方法:将MRL小鼠分为复方中药组、桂枝组、强的松组和空白对照组,检测MRL小鼠心肌组织凋亡细胞,计算心肌细胞凋亡指数(AI),RT-PCR法检测心肌细胞凋亡基因Bax mRNA、Caspase 3 mRNA表达,观察复方中药(组成:丹参、桂枝、西洋参、冰片)对MRL小鼠心肌细胞凋亡的影响.结果:复方中药能抑制Bax mRNA、Caspase 3 mRNA表达,明显抑制心肌细胞凋亡(心肌细胞凋亡指数显著下降).其疗效明显优于桂枝组和空白对照组(P<0.05),与强的松组疗效相当,P>0.05.结论:复方中药可能通过调控凋亡基因Bax mRNA、Caspase 3 mRNA表达而抑制心肌细胞凋亡,从而延缓或部分逆转MRL心肌损害的发生、发展.
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| 关 键 词： | 系统性红斑狼疮 MRL小鼠 细胞凋亡 Bax mRNA、Caspase 3 mRNA基因表达 复方中药 |
Effect of complex prescription of Chinese medicine on myocardial cell apoptosis and expression of Bax mRNA and Caspase 3 mRNA gene in MRL mice |
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| LIU Guijing,YAN Yingjie,MIAO Guoying,CHEN Xingqi,YU Xue,ZHANG Xiangling,LI Zengyan,WANG Zhenyu. Effect of complex prescription of Chinese medicine on myocardial cell apoptosis and expression of Bax mRNA and Caspase 3 mRNA gene in MRL mice[J]. Journal of Clinical Cardiology, 2011, 27(7) |
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| Authors: | LIU Guijing YAN Yingjie MIAO Guoying CHEN Xingqi YU Xue ZHANG Xiangling LI Zengyan WANG Zhenyu |
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| Affiliation: | LIU Guijing1 YAN Yingjie1 MIAO Guoying1 CHEN Xingqi1 YU Xue1 ZHANG Xiangling1 LI Zengyan1 WANG Zhenyu2(1Affiliated Hospital of Hebei Engineering University,Hebei Handan,056029,China,2Xinxing Zhuguan Staff Hospital) |
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| Abstract: | Objective:To study the effect of complex prescription of Chinese medicine on myocardial cell apoptosis and expression of Bax mRNA and Caspase 3 mRNA gene in MRL mice.Method:MRL mice were divided into complex prescription of Chinese medicine group,Vacant group,Guizhi group,Prednisone group.Detect myocardial apoptosis cell of MRL mice and calculate Myocardial cell Apoptosis index(AI).Detect expressions of Bax mRNA and Caspase 3 mRNA gene with the experimental method of RT-PCR and observe the effect of complex... |
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| Keywords: | MRL mice myocardial cell apoptosis expression of Bax mRNA and Caspase 3 mRNA Gene |
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