| 人苍白杆菌改良头孢哌酮麦康凯培养基分离 |
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| 引用本文： | 华德,王少玲,胡伟,吴捷.人苍白杆菌改良头孢哌酮麦康凯培养基分离[J].中国公共卫生,2011,27(4). |
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| 作者姓名： | 华德 王少玲 胡伟 吴捷 |
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| 作者单位： | 海南省疾病预防控制中心微生物检验所,海口,570203 |
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| 摘 要： | 目的 了解人苍白杆菌形态、培养、生化及药敏等特性,建立对人苍白杆菌选择性的分离方法.方法 用改良头孢哌酮麦康凯平板对人苍白杆菌分离,API 20 E和VITEC2GN进行生化鉴定,AST-GN 10进行药敏测定.结果 在206份检样中分离出21株人苍白杆菌;人苍白杆菌生化特点是对糖和醇类不利用;替卡两林、哌拉西林等抗生素对人苍白杆菌的MIC≥128;头孢类抗生素的MICI≥64;四环素、左氧氟沙星、环丙沙星、美罗培南、亚胺培南等的MIC均≤1.结论 改良头孢哌酮麦康凯平板对人苍白杆菌分离具很高的选择性,适合自然环境和临床检验中对人苍白杆菌分离鉴定;人苍白杆菌对β-内酰胺类抗生素耐药性较高.
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| 关 键 词： | 人苍白杆菌 改良头孢哌酮麦康凯 分离鉴定 |
Isolation of Ochrobactrum anthropi with modified cefoperazone Mac-Lonkey agar |
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| HUA De,WANG Shao-ling,HU Wei,et al..Isolation of Ochrobactrum anthropi with modified cefoperazone Mac-Lonkey agar[J].Chinese Journal of Public Health,2011,27(4). |
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| Authors: | HUA De WANG Shao-ling HU Wei |
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| Institution: | HUA De,WANG Shao-ling,HU Wei,et al. Department of Microorgnism Detection,Hainan Provincial Center for Disease Control and Prevention(Haikou 570203,China) |
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| Abstract: | Objective To know the morphology,culture,biochemistry,and drug sensitivity of Ochrobactrum anthropi and to establish a suitable methods for its identification. Methods Ochrobactrum anthropi was isolated with modified Mac-Lonkey agar(with cefoperazone added) and identified by AP I 20E,VITEC2 GN.The drug sensitivity of Ochrobactrum anthropi was determined with AST-GN10. Results From 206 samples,there were 21 Ochrobactrum anthropi strains isolated.The isolated strains could not utilize glucose and alcohol.The ... |
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| Keywords: | Ochrobactrum anthropi modified Mac-lonkey agar method isolation and identification |
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