| 丹皮酚对大鼠离体海马神经元NMDA受体的影响 |
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| 引用本文： | 武继彪,隋在云,管华诗. 丹皮酚对大鼠离体海马神经元NMDA受体的影响[J]. 中药药理与临床, 2007, 23(5): 71-73 |
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| 作者姓名： | 武继彪 隋在云 管华诗 |
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| 作者单位： | 1. 中国海洋大学,青岛,266003
2. 山东省中医药研究院,济南,250014 |
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| 摘 要： | 目的:研究丹皮酚对大鼠海马神经元缺糖缺氧的保护作用。方法:以原代培养的新生大鼠海马神经元作为研究对象,建立缺糖缺氧损伤模型,倒置相差显微镜下进行一般形态学的动态观察,用MTT法测定神经元存活率。通过放射配基结合实验测定海马NMDA受体结合力变化。结果:丹皮酚可显著降低神经元缺糖缺氧时细胞死亡率,减弱NMDA受体结合力。结论:丹皮酚对离体培养的大鼠海马神经元缺糖缺氧具有保护作用,其作用机制与减弱NMDA受体结合力有关。
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| 关 键 词： | 丹皮酚 海马神经元 NMDA受体 |
Effects of peaonol on NMDA receptor in cultured rat hippocampal neurons |
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| Wu Jibiao,Sui Zaiyun,Guan Huashi. Effects of peaonol on NMDA receptor in cultured rat hippocampal neurons[J]. Pharmacology and Clinics of Chinese Materia Medica, 2007, 23(5): 71-73 |
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| Authors: | Wu Jibiao Sui Zaiyun Guan Huashi |
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| Affiliation: | 1 School of Medicine ,China Ocean University, Qingdao 266003 ;2 Shandong Academy of TCM, Jinan 250014 |
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| Abstract: | Objective: To determine if peaonol can protect cultured rat hippocampal neurons from anoxia-induced injury.Methods: We cultivate the rat hippocampal neurons as the object of study and then build the model of oxygen-glucose deprivation.They were randomly divided into 3 groups.Each group was observed by inverted phase contrast microscope;bonding force of NMDA receptors was evaluated by liquid scintillation counting.Results: Compared with OGD group at each designated period,peaonol treatment after OGD obviously increased cell survival rate and reduced the bonding force of NMDA receptors.Conclusion: Peaonol can protect rat neurons from ischemia-reperfusion injury by alleviating morphological damage and increasing neuron viability. |
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| Keywords: | peaonol hippocampal neurons NMDA receptor |
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