| 黄芩苷对人口腔上皮癌KB细胞端粒酶活性和细胞周期的影响 |
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| 引用本文： | 李宏捷,谢文利,朱江.黄芩苷对人口腔上皮癌KB细胞端粒酶活性和细胞周期的影响[J].中药药理与临床,2007,23(4):19-21. |
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| 作者姓名： | 李宏捷 谢文利 朱江 |
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| 作者单位： | 1. 天津医科大学口腔医学院口腔病理教研室,天津,300070
2. 武警医学院药理教研室,天津,300162 |
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| 摘 要： | 目的:研究黄芩苷(bacailin)对KB细胞生长抑制作用及其对端粒酶活性和细胞周期的影响。方法:采用MTT法检测bacailin对体外培养的KB细胞增殖的抑制作用,TRAP-PCR-ELISA方法检测KB细胞在bacailin作用后端粒酶活性的变化,用流式细胞仪分析细胞周期的改变。结果:bacailin在0.5～2.0mmol/L剂量范围内对KB细胞有抑制作用,且呈剂量依赖关系(P<0.05),同时bacailin作用后,KB细胞端粒酶活性明显抑制(P<0.05),KB细胞的G2/M期细胞含量明显增高(P<0.01)。结论:bacailin对肿瘤细胞的端粒酶活性有抑制作用。
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| 关 键 词： | 黄芩苷 端粒酶 肿瘤细胞 |
The effects of bacailin on telomerse activity and cells phases in KB tumor cells |
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| Li HongJie,Xie Wenli,Zhu Jiang.The effects of bacailin on telomerse activity and cells phases in KB tumor cells[J].Pharmacology and Clinics of Chinese Materia Medica,2007,23(4):19-21. |
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| Authors: | Li HongJie Xie Wenli Zhu Jiang |
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| Institution: | Department of Oral Pathology, College of Stomatology, Tianjin Medical University, Tianjin 300070 |
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| Abstract: | Objective: To study the inhibitory effect of bacailin to KB tumor cells , and its influences on telomerse activity and cells phases in KB . Methods: The inhibitory effect of bacailin on KB tumor cells proliferation was assayed by MTT method; telomerse activity was determined by TRAP-PCR-ELISA in untreated and treated KB tumor cells by bacailin . Results: Bacailin (0.5~2.0mmol/L) inhibited the proliferation of KB tumor cells in vitro, antitumor effect of it was in a dose-dependent manner;Telomerse activity of KB was significantly inhibited when treated with bacailin, cell cycle of treated KB tumor cells were marked increase in G2/M phase. Conclusion: Bacailin can effectively inhibit telomerse activity of tumer cells. |
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| Keywords: | bacailin telomerse tumor cells |
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