谷胱甘肽-S-转移酶M3基因-63A/C多态性与原发性高血压的相关性

目的研究谷胱甘肽-S-转移酶(glutathione S-transferase M3,GSTM3)基因-63A/C多态性在中国北方汉族中的分布及其与原发性高血压(essential hypertension,EH)的关联。方法应用聚合酶链反应-限制性片段长度多态性技术,对汉族234例EH患者及328名正常人GSTM3基因-63A/C位点多态性进行基因分型,随机选择部分基因型样品进行DNA测序验证。结果GSTM3基因-63A/C位点基因型分布在EH和对照组均符合Hardy-Weinberg遗传平衡。EH组CC基因型频率(6%)显著高于对照组(1.8%)(P<0.05),Logistic回归分析显示CC基因型是EH发生的一项独立危险因素(OR=3.447,95%CI:1.19～7.63;P=0.04)。结论GSTM3基因-63A/C多态性与EH相关,C等位基因可能是中国北方汉族人群EH的易感性标志。

Association of - 63A/C polymorphism of glutathione S-transferase M3 gene with essential hypertension in Chinese population

Objective To investigate the distribution of-63A/C polymorphism of human glutathione S-transferase M3(GSTM3) gene in Chinese Han population and the association of-63A/C polymorphism with essential hypertension(EH).Methods-63A/C polymorphism of GSTM3 gene was examined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 234 patients with EH and 328 healthy controls.The genotypes were confirmed partially by DNA sequencing.Results The distribution of GSTM3-63A/C was in Hardy-Weinberg equilibrium.The CC genotype frequency in EH group(6%) was significantly higher than that in control group(1.8%)(P<0.05).After adjustment for age,body mass index,and other risk factors,the CC genotype was independently associated with hypertension(OR=3.447,95%CI:1.19-7.63;P=0.04).Conclusion The GSTM3-63A/C polymorphism was associated with EH in Chinese Han population,the C allele might be a risk factor for EH in Chinese Han nationality.