| 浙江汉族人群KIR2DL3基因遗传多态性 |
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| 引用本文： | 朱发明,姜侃,吕沁风,章伟,何吉,许先国,严力行. 浙江汉族人群KIR2DL3基因遗传多态性[J]. 中华医学遗传学杂志, 2007, 24(1): 88-90 |
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| 作者姓名： | 朱发明 姜侃 吕沁风 章伟 何吉 许先国 严力行 |
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| 作者单位： | 310006,杭州,浙江省血液中心,卫生部血液安全重点研究实验室 |
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| 基金项目： | 浙江省自然科学基金(Y204216)，浙江省医药卫生科学研究基金(2004A014)~~ |
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| 摘 要： | 目的探讨浙江汉族人群KIR2DL3基因的多态性分布情况。方法采用PCR测序分型方法进行KIR2DL3等位基因分型。首先合成引物特异性扩增KIR2DL3基因的两个片段,然后对KIR2DL3基因第4、5、7、8和9外显子进行测序分析,根据多态性位点格局判断样本的KIR2DL3等位基因情况。结果样本中共检测到两种等位基因,其中KIR2DL3*001等位基因占77%。结论PCR测序分型方法可有效检测KIR2DL3等位基因。
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| 关 键 词： | 杀伤细胞免疫球蛋白样受体2DL3基因 基因频率 PCR测序分型 |
| 修稿时间： | 2006-06-27 |
Polymorphism of killer cell immunoglobulin-like receptor 2DL3 gene in Zhejiang Han population |
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| ZHU Fa-ming,JIANG Kan,LV Qin-feng,ZHANG Wei,HE Ji,XU Xian-guo,YAN Li-xing. Polymorphism of killer cell immunoglobulin-like receptor 2DL3 gene in Zhejiang Han population[J]. Chinese journal of medical genetics, 2007, 24(1): 88-90 |
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| Authors: | ZHU Fa-ming JIANG Kan LV Qin-feng ZHANG Wei HE Ji XU Xian-guo YAN Li-xing |
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| Affiliation: | Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 P. R. China |
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| Abstract: | Objective To analyze the allelic polymorphism of killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) gene in Zhejiang Han population. Methods Allelic of KIR2DL3 was detected by PCR sequence based typing (PCR-SBT) method. Two specific fragments of KIR2DL3 were amplified using different primers and sequenced for exons 4,5,7,8,9 of KIR2DL3. All the samples would be assigned to KIR2DL3 allele according to the polymorphism site patterns. Results Two KIR2DL3 alleles were observed, with KIR2DL3*001 having higher frequency, 0.77. Conclusion The PCR-SBT method is reliable and there are distinctive frequencies of KIR2DL3 alleles in Zhejiang Han population. |
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| Keywords: | killer cell immunoglobulin-like receptor 2DL3 gene gene frequency PCR-sequence based typing |
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