急性和慢性EB病毒感染儿童外周血CD8+T 细胞活化差异

摘要 目的：比较急性和慢性EB病毒感染儿童总T细胞，CD8+T细胞及CD8+T 细胞活化HLA-DR表达的差异，探讨不同感染形式的不同发病机制。方法：应用流式细胞术检测27例急性EB病毒感染儿童，7例慢性EB病毒感染儿童和29例对照组儿童外周血淋巴细胞中总T细胞，CD8+T细胞和CD8+T细胞活化HLA-DR表达水平，并对急性和慢性感染儿童各项检测指标之间进行比较。 结果：急性EB病毒感染儿童总T细胞，CD8+T细胞， CD8+/HLA-DR+细胞明显高于慢性EB病毒感染儿童和对照组（p<0.01）；慢性EB病毒感染儿童总T细胞低于对照组，CD8+/HLA-DR+细胞高于对照组（p<0.01）。结论：急性和慢性EB病毒感染儿童总T细胞，CD8+T细胞及CD8+T 细胞活化HLA-DR表达存在明显差异，慢性EB病毒感染儿童CD8+T 细胞处于低水平活化状态，CD8+T细胞活化水平检测可作为评估EB病毒感染患儿细胞免疫功能的有效指标。

Difference in CD8+T cell activation of prepheral blood between acute and chronic EB virus infection children

Abstract Objective:To analyze the difference in total T cell, CD8+T cell and HLA-DR expression on CD8+T cell of peripheral blood in 27 children with acute EB virus infection and 7 children with chronic EB virus infection.Results: Total T cell , CD8+T cell and CD8+/HLA-DR+ cell in acute infected group were higher than chronic infected group and control(p<0.01);T cell was lower than control and CD8+/HLA-DR+ cell was higher than control in chronic infected group(p<0.01).Conclusion: There were significant difference in total T cell, CD8+T cell and HLA-DR expression on CD8+T cell of peripheral blood between acute and chronic EB virus infection ,It was low activated level of CD8+T cell in chronic EB virus infection.It can evaluated cellular immune function to detecte CD8+T cell activation for EB virus infected children.