P53、VEGF在SKOV3荷瘤裸鼠不良心理应激模型血清及移植瘤中的表达及意义

目的:探讨不良心理应激对SKOV3荷瘤裸鼠血清及移植瘤中P53、血管内皮生长因子(VEGF)蛋白水平的影响。方法:建立荷卵巢癌裸鼠不良心理应激模型,采用ELISA检测正常对照组(A)、单纯应激组(B)、单纯荷瘤组(C)和荷瘤应激组(D)各10只小鼠的血清P53和VEGF浓度,用Western blot方法分析C组和D组移植瘤中P53、VEGF蛋白表达情况;另取32例荷瘤应激和24例单纯荷瘤的裸鼠移植瘤标本,免疫组化检测P53和VEGF的蛋白表达水平。结果:A组与B组血清P53和VEGF含量比较差异无统计学意义(P>0.05),C组的P53、VEGF含量均明显高于A组(P<0.01),D组的血清P53、VEGF与B、C组相比差异均有统计学意义(P<0.05或P<0.01);D组P53、VEGF的蛋白灰度值明显高于C组(P<0.05);免疫组化结果示P53蛋白在荷瘤应激组和单纯荷瘤组的阳性表达率分别为87.5%和62.5%,VEGF蛋白在2组的阳性表达率分别为93.8%和70.8%,差异均有统计学意义(P<0.05)。结论:不良心理应激明显促进荷瘤鼠SKOV3移植瘤生长,可能与P53和VEGF有关;P53及VEGF有望成为心理肿瘤学的分子靶标。

Expression of P53 and VEGF in serum and xenograft of SKOV3 tumor-bearing nude mice model of adverse psychological stress and their significances

purpose： To investigate the effects of adverse psychological stress on p53 and VEGF protein levels in serum and xenograft of SKOV3 tumor-bearing nude mice. Methods: The adverse psychological stress model of nude mice bearing ovarian cancer were established, the levels of P53, VEGF in serum were detected by ELISA in normal control, simple stress, simple tumor and tumor-bearing stress group, immunohistochemistry and Western Blot were performed to detect the expression of P53, VEGF protein for xenografts in the tumor group and tumor-bearing stress group. Results : The levels of P53 and VEGF in serum of stress groups were higher than those in corresponding group that not subjected to stress, the differences were significant (P <0.05). Western Blot showed that the average density value ratio of P53 and VEGF in tumor-bearing stress group were 2.163 ± 0.072 and 2.523 ± 0.088, respectively, while those in simple tumor group were 1.116 ± 0.068 and 1.477 ± 0.083, respectively, the differences were significant (P <0.01).The results of immunohistochemistry showed that P53 protein in tumor-bearing stress group (n = 32) and tumor group (n = 24) positive expression rates were 87.5% and 62.5%, while VEGF protein were 93.8% and 70.8%, the differences were significant (P<0.05). Conclusion: adverse psychological stress may accelerate the progress of SKOV3 tumor, the expression of P53 and VEGF may be concerned in the mechanism; P53 and VEGF are expected to become the molecular targets for psycho-oncology study.