CA125及CA19-9抗原在子宫内膜异位症组织中的表达及意义

目的：探讨CA125及CA19-9抗原在子宫内膜异位症子宫内膜组织中的表达意义及其与血清值之间的相关关系。 方法：应用免疫组织化学S-P法观察20例子宫内膜异位症患者异位子宫内膜（卵巢）及其在位子宫内膜CA125及CA19-9的蛋白质表达；应用磁性分离酶联免疫法测定20例子宫内膜内异症患者血清CA125及CA19-9的含量。 结果：CA125及CA19-9蛋白质主要表达于异位子宫内膜腺体上皮细胞。CA125蛋白质在异位子宫内膜及在位子宫内膜的阳性表达率分别为90.0%及25.0%；CA19-9蛋白质在异位子宫内膜及在位子宫内膜的阳性表达率分别为85.0%及10.0%。6例Ⅰ、Ⅱ期子宫内膜异位症患者血清CA19-9、CA125值（U?mL^-1）及阳性率分别为(29.64±10.24)、(25.85±9.45) 及16.7%、33.3%。14例Ⅲ、Ⅳ期子宫内膜异位症患者血清CA19-9、CA125值（U?mL^-1）及阳性率分别为(46.54±14.29)、（58.02±20.11）及42.8%、71.4%。异位内膜CA19-9免疫组化染色阳性率为85.0%（17/20），与血清中CA19-9测定值之间呈正相关关系（r_s=0.36,P<0.05）。 结论：CA125及CA19-9蛋白质在子宫内膜异位症患者的异位子宫内膜呈高表达，而在在位子宫内膜呈低表达；子宫内膜异位症患者血清中CA19-9主要来源于异位子宫内膜。

Expressions of CA125 and CA19-9 in tissues of patients with endometriosis and their significances

Objective To investigate the expressions of CA125 and CA19-9 in endometriosis and the relationship with the serum values of CA125 and CA19-9. Methods Serum CA125 and CA19-9 levels were measured by using the magnetic separated enzyme linked immunosorbent assay respectively in 20 patients with sendometriosis. Forty paraffin samples of endometriosis(20 ectopic endometria and 20 endometria)were examined by immunohistochemical staining method to detect the expressions of CA125 and CA19-9. Results CA125 and CA19-9 were mainly expressed in the cytoplasm of glandular epithelial cells. The positive expression rates of CA125 in ectopic endometrium and endometrium were 90.0% and 25.0%, respectively;The expression rates of CA19-9 in ectopic endometrium and endometrium were 85.0% and 10.0%, respectively. The mean serum values of CA19-9 and CA125(U·mL~ -1)in 6 patients with stage Ⅰ and Ⅱ endometriosis were (29.64±10.24) and (25.85±9.45),respectively; the positive rates were 16.7% and 33.3%,respectively. The mean serum value of CA19-9 and CA125 (U·mL~ -1) in 14 patients with stage Ⅲ and Ⅳ endometriosis were (46.54±14.29 ) and (58.02±20.11), respectively;the positive rates were 42.8% and 71.4%, respectively. The expression of CA19-9 in ectopic endometrium was correlated to the serum value of CA19-9 (r s=0.36,P<0.05), but not CA125 (r s=0.12,P>0.05). Conclusion The CA125 and CA19-9 proteins express highly in ectopic endometrium, and express lowerly in endometrium. The main resource of serum CA19-9 in patients with endometriosis is ectopic endometrium.