增生性玻璃体视网膜病变模型眼内定向 转染报告基因的研究

目的观察目的基因在玻璃体增生膜的表达情况，以探讨基因治疗增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR) 的可行性。方法用β-半乳糖苷酶基因作为报告基因，以逆转录病毒载体携带，直接注入PVR模型眼玻璃体腔中，观察其在PVR 眼各组织表达情况。结果 基因转染后可见玻璃体增生膜组织有转染基因表达，表达主要位于增生膜的表面，而视网膜组织及其它眼组织未见表达。结论逆转录病毒载体用于PVR的基因治疗有靶向性作用，表明PVR基因治疗具有可行性。(中华眼底病杂志,2001,17:224-226)

Experimental study on targeting transfer of reporter gene in proliferative vitreoretinopathy of rabbit

PurposeTo study the possibility of prevention of proliferative vitreoretinopathy（PVR） by transduction of exogenous gene in vivo．MethodsPVR model of rabbits was induced by intravitreal injection of fibroblasts．β-galactosidase (lacZ) gene as a reporter gene was transfered into the vitreous of PVR model eyes mediated by retroviral vector, and the expression of the gene in eye tissues was determined ． Gene transfection was done on the 6th day after fibroblasts injection,and the dosage of intravitreal injection of reporter gene was 0．1ml PLXSN/lacZ serum-free supernatant (1．1×106 cfu/ml)．ResultslacZ gene expression was seen in proliferative membranes after gene transfection, and the expression was located maily at the surface of PVR membrane．The reporter gene expression lasted at least more than 30 days．No expression was found in retinal tissues．ConclusionsRetrovirus mediated gene can be directionally transducted in PVR membrane,and might possess the feasibility of gene therapy for PVR．(Chin J Ocul Fundus Dis, 2001,17:224-226)