橙皮苷对去卵巢骨质疏松症大鼠骨质流失和机制的影响

目的研究橙皮苷对去卵巢骨质疏松大鼠骨质流失和骨保护素(osteoprotegerin,OPG)/核因子kappa B配体的受体激活物(receptor activator of nuclear factor kappa B ligand,RANKL)/RANK通路的影响。方法将48只雌性Sprague-Dawley(SD)大鼠随机分为假手术组、去卵巢骨质疏松组、橙皮苷低剂量组、橙皮苷中剂量、橙皮苷高剂量组和雌激素组。检测骨组织骨体积分数(bone volume fraction,BV/TV)、骨小梁厚度(trabecular thickness,Tb.Th)、骨密度(bone mineral density,BMD)、骨小梁数量(trabecular number,Tb.N)、骨小梁分离度(trabecular separation,Tb.Sp),通过HE染色观察骨组织病理损伤,采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测人Ⅰ型胶原C端肽(C-terminal peptide collagen typeⅠ,CTX-Ⅰ)和血清骨钙素(bone glaprotein,BGP)的表达量。运用qRT-PCR和Western bolt分别检测骨组织OPG、RANKL、RANK的mRNA和蛋白表达。结果在橙皮苷治疗后,与去卵巢骨质疏松组相比,骨质疏松标志物BV/TV、BMD、Tb.Th和Tb.N水平升高,Tb.Sp水平降低;大鼠骨小梁数量明显增加,且骨小梁连接更为紧密;骨吸收标志物CTX-Ⅰ水平降低;骨形成标志物BGP水平升高。OPG mRNA相对表达量和蛋白的表达量升高,RANKL和RANK mRNA相对表达量和蛋白的表达量降低。结论橙皮苷能缓解去卵巢骨质疏松大鼠骨质流失并调节OPG/RANKL/RANK信号通路。

Effect of hesperidin on bone loss and mechanism in ovariectomized osteoporosis rats

Objective To study the effects of hesperidin on bone loss and osteoprotegerin (OPG)/receptor activator of nuclear factor kappa B ligand (RANKL)/ RANK signaling pathway in ovariectomized osteoporotic rats. Methods Forty-eight female Sprague-Dawley (SD) rats were randomly divided into sham operation group, ovariectomized osteoporosis group, hesperidin low-dose group, hesperidin middle-dose group, hesperidin high-dose group, and estrogen group. Bone volume fraction (BV/TV), bone mineral density (BMD), trabecular thickness (Tb.Th), trabecular number (Tb.N) ), and trabecular separation (Tb.Sp) were detected. The histopathology of bone tissue was observed with HE staining. C-terminal peptide collagen type I (CTX-I) and bone glaprotein (BGP) were detected with enzyme-linked immunosorbent method (ELISA). The mRNA and protein expression of OPG, RANKL, and RANK were detected with qRT-PCR and Western blotting in bone tissues. Results After treatment with hesperidin, the osteoporosis markers BV/TV, BMD, Tb.Th, and Tb.N increased, and Tb.Sp decreased compared to those in ovariectomized osteoporosis group. The number of trabecular bones in rats significantly increased, and the trabecular bone connections were tighter. The bone resorption marker CTX-I decreased, and the bone formation marker BGP increased. The relative mRNA and protein expression of OPG increased, while the relative mRNA and protein expression of RANKL and RANK decreased. Conclusion Hesperidin alleviates bone loss in ovariectomized osteoporosis rats and regulates OPG/RANKL/RANK signaling pathway.