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Isolation,structure determination and synthesis of a novel tryptophan-containing heptapeptide (basic tryptophyllin) from the skin of Phyllomedusa rohdei |
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| Authors: | PIER CARLO MONTECUCCHI MARCO VINCENTI ANNA MARIA LAZZARINI LUISA RUSCONI VITTORIO ERSPAMER |
| Abstract: | A novel tryptophyllin has been isolated from the methanol extracts of the skin of the South American frog Phyllomedusa rohdei. Its primary structure, as determined by chemical/enzymatic methods and by FAB mass spectrometry, is the following: Lys-Pro-Hyp-Ser-Trp-Ile-Pro-NH2. This basic tryptophyllin has been synthesized in solid phase, in order to allow its complete pharmacological characterization. |
| Keywords: | automatic Edman degradation FAB frog skin gas-phase sequencer trans-4-hydroxyproline mass spectrometry PTH Hyp Phyllomedusa rohdei solid-phase peptide synthesis tryptophyllins |