石榴补血糖浆对血虚小鼠造血功能的影响＊

目的 探讨石榴补血糖浆对环磷酰胺血虚证模型小鼠造血功能的影响及其相关机制。方法 采用环磷酰胺构建血虚证模型,分别用石榴补血糖浆、EPO和复方阿胶浆连续干预28d,取小鼠外周血液,分别进行红细胞（RBC）及白细胞（WBC）计数、血红蛋白（HGB）浓度测定以及红细胞比容（HCT）,血小板计数（PLT）的含量;取小鼠双侧股骨,收集全部骨髓细胞,进行骨髓有核细胞计数;采用酶联免疫法（Elisa）测定小鼠血清中促红细胞生成素（Erythropoietin,EPO）的含量,Western blot、Real-Time PCR法检测造血干细胞因子（SCF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）的含量变化。结果 与对照组比较,石榴补血糖浆（4.6875 mL·kg^-1,9.375 mL·kg^-1,18.75 mL·kg^-1,28.125 mL·kg^-1）组小鼠血液中RBC,WBC,PLT,HCT,HGB均升高（P < 0.05）;与模型组相比,石榴补血糖浆9.375 mL·kg^-1剂量组EPO、SCF、GM-CSF表达量均显著提升（P < 0.05）,骨髓有核细胞数增多（P < 0.05）。结论 石榴补血糖浆可以对血虚证模型小鼠起到一定的治疗作用,其作用机理可能与增加造血细胞因子EPO、SCF、GM-CSF的表达,提高骨髓有核细胞数有关。

Effect of Shiliu Buxue Granule on Hematopoietic Function in Mice with Blood Deficiency Syndrome

Objective To investigate the effect of Shiliu Buxue Granule on hematopoietic function in mice with blood deficiency syndrome induced by cyclophosphamide and its related mechanism.Methods Cyclophosphamide was used to construct the model of blood deficiency syndrome. The contents of HCT, RBC, HGB, WBC and PLT in peripheral blood of mice were detected. All bone marrow cells were collected from bilateral femurs of mice. The number of bone marrow nucleated cells was counted. The content of erythropoietin (EPO) in serum of mice was determined by enzyme-linked immunosorbent assay (ELISA). The contents of SCF and GM-CSF were measured by the WB and PCR.Results Compared with the control group, RBC, WBC, PLT, HCT and HGB in the blood of mice in Shiliu Buxue Granule group (4.6875 mL·kg^-1, 9.375 mL·kg^-1, 18.75 mL·kg^-1, 28.125 mL·kg^-1) were all increased (P < 0.05). Compared with the model group, the expression of EPO, SCF, GM-CSF in Shiliu Buxue Granule group were significantly increased (P < 0.05), and the number of nucleated cells in the bone marrow increased (P < 0.05).Conclusion Shiliu Buxue Granules can play a therapeutic role in mice with blood deficiency syndrome, and its mechanism may be related to increasing the expression of hematopoietic cytokines EPO, SCF, GM-CSF and increasing the number of bone marrow nucleated cells.