人放射性皮肤溃疡c-erbB-2、EGFR蛋白高表达病理研究

我们应用ｃ－ｅｒｂＢ－２、ＥＧＦＲ蛋白抗体和微波炉抗原修复法对２５例人放射性皮肤溃进行免疫组织化学研究。结果发现ｃ－ｅｒｂＢ－２癌蛋白高表达阳性率为９２．０％（２３／２５），ＥＧＦＲ蛋白高表达阳性率为７３．７％（１４／１４）；二者阳性部位一致，主要见于鳞状上皮细胞膜、间质纤维母细胞、血管内皮细胞以及小动脉中层平滑肌细胞和外层纤维细胞胞质，提示ｃ－ｅｒｂＢ－２、ＥＧＦＲ蛋白的高表达可能与放射性皮肤溃

A pathological study on overexpression of c-erbB-2,EGFR proteins in human radiation skin ulcer

We performed an immunohistochemical study in 25 cases of human radiation skin ulcer by using c-erbB-2, Epidermal growth factor receptor(EGFR), protein antibodies and repairing method of antigen with microwave oven.The results show that the positive rate for overexpression of c-erbB-2 oncoprotein was 92.0% (23/25), and EGFR was 73.7% (14/19).The positive position for overexpression of c-crbB-2 was mainly observed on cell memberane of squamous epithelial cells and cell plasma of fibroblasts, endotheliocytes, and leiomyocytes in media and fibrocytes in adventitia of the arterioles in mesenchyme.It is suggested that the overexpression of c-erbB-2 and EGFR proteins might be related to cancer transformation and poor healing in radiation skin ulcers.