374例系统性红斑狼疮患者自身抗体检测及结果分析

目的 探讨血清自身抗体检测对系统性红斑狼疮（SLE）的诊断价值和临床意义.方法 采用间接免疫荧光法（IIFA）检测SLE患者血清中的抗核抗体（ANA）;抗ds-DNA抗体的检测采用免疫斑点金渗滤法;采用酶免疫斑点法检测6种特异性核抗原的自身抗体（抗Sm、RNP、SS-A、SS-B、Scl-70和JO-1抗体）.结果 ①374例SLE患者中,IIFA检测ANA阴性31例,ANA阳性者343例（阳性率91.7%）,主要荧光模式为核颗粒型、核颗粒/均质型;②抗Sm、RNP、SS-A、SS-B、Sc1-70、Jo-1、dsDNA的阳性率分别为15.0%、23.8%、50.5%、12.0%、0%、0.3%和43.8%.结论 ANA的联合检测对SLE的诊断和疗效观察具有重要意义.

Analysis of Autoantibody Profiles of 374 Cases of Systemic Lupus Erythematosus

Objective To explore the diagnostic value and clinical significance of autoantibody test for SLE patients. Methods Serum samples of 374 SLE patients were analyzed by detecting antinuclear antibody （ ANA）, anti-ds-DNA antidody and ENA antibody repertoire（ anti-Sm, anti-RNP, anti-SS-A, anti-SS-B, anti- Sc170 and anti-Jol antibody）with indirect immunofluorescence assay（IIFA） and gold linked immunodotting percolation and enzyme linked immunodotting respectively. Results （~Among the 374 SLE patients, there were 31 cases of ANA negative of IIFA test, and the positive rate of ANA （IIFA）for SLE patients was 91.7% ,and speckled pattern and speckled/homogeneuous pattern were the major immunofluorescence pat- terns ; （~the positive rate of anti-Sm antibody, anti-RNP antibody, anti-SS-A antibody, anti-SS-B antibody, anti-Scl70 antibody,anti-Jol antibody and anti-dsDNA were 15.0% ,23.8% ,50.5%, 12.0% ,0% ,0.3% and 43.8%. Conclusion Co-detection has great clinical significance for diagnosis and evaluation of cura- tive effect of SLE.